sp. nov., isolated from nodules of in Spain No Access

Abstract

Two endophytic strains, coded MOVP5 and MOPV6, were isolated from nodules of plants grown on agricultural soil in Southeastern Spain, and were characterized through a polyphasic taxonomy approach. Their 16S rRNA gene sequences showed 99.3 and 99.4 %, 98.9 and 99.6 %, and 99.0 and 98.7% similarity to ‘’ YIC 4121, LGM 140, and NRCPB10, respectively. Multilocus sequence analysis based on sequences of and genes suggested that these two strains could represent a new species with less than 96.5 % similarity to their closest relatives. PCR amplification of the gene, involved in synthesis of protelomerase, confirmed the affiliation of strains MOPV5 and MOPV6 to the genus . Whole genome average nucleotide identity and digital DNA–DNA hybridization average values were less than 95.1 and 66.7 %, respectively, with respect to its closest related species. Major fatty acids in strain MOPV5 were Cω7/Cω6 in summed feature 8, C cyclo ω8, C and C 3-OH. Colonies were small to medium, pearl-white coloured on YMA at 28 °C and growth was observed at 10–42 °C, pH 5.0–10.0 and with 0.0–0.5 % (w/v) NaCl. The DNA G+C content was 59.9 mol%. These two strains differ from all other genomovars of found so far, including those that have not yet given a Latin name. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strain MOPV5 as representing a novel species of , for which the name sp. nov. is proposed. The type strain is MOPV5 (=CECT 30096=LMG 31779).

Funding
This study was supported by the:
  • European Regional Development Fund (Award CLU-2019-05 – IRNASA/CSIC Unit of Excellence)
    • Principle Award Recipient: ÁlvaroPeix
  • European Regional Development Fund (Award AGL2017–85676R)
    • Principle Award Recipient: EulogioJ. Bedmar
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2021-12-06
2024-03-28
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