1887

Abstract

Two endophytic strains, coded MOVP5 and MOPV6, were isolated from nodules of plants grown on agricultural soil in Southeastern Spain, and were characterized through a polyphasic taxonomy approach. Their 16S rRNA gene sequences showed 99.3 and 99.4 %, 98.9 and 99.6 %, and 99.0 and 98.7% similarity to ‘’ YIC 4121, LGM 140, and NRCPB10, respectively. Multilocus sequence analysis based on sequences of and genes suggested that these two strains could represent a new species with less than 96.5 % similarity to their closest relatives. PCR amplification of the gene, involved in synthesis of protelomerase, confirmed the affiliation of strains MOPV5 and MOPV6 to the genus . Whole genome average nucleotide identity and digital DNA–DNA hybridization average values were less than 95.1 and 66.7 %, respectively, with respect to its closest related species. Major fatty acids in strain MOPV5 were Cω7/Cω6 in summed feature 8, C cyclo ω8, C and C 3-OH. Colonies were small to medium, pearl-white coloured on YMA at 28 °C and growth was observed at 10–42 °C, pH 5.0–10.0 and with 0.0–0.5 % (w/v) NaCl. The DNA G+C content was 59.9 mol%. These two strains differ from all other genomovars of found so far, including those that have not yet given a Latin name. The combined genotypic, phenotypic and chemotaxonomic data support the classification of strain MOPV5 as representing a novel species of , for which the name sp. nov. is proposed. The type strain is MOPV5 (=CECT 30096=LMG 31779).

Funding
This study was supported by the:
  • European Regional Development Fund (Award CLU-2019-05 – IRNASA/CSIC Unit of Excellence)
    • Principle Award Recipient: ÁlvaroPeix
  • European Regional Development Fund (Award AGL2017–85676R)
    • Principle Award Recipient: EulogioJ. Bedmar
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2021-12-06
2022-01-29
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