1887

Abstract

is an important genus, with several species associated with infections in humans and animals. In a survey of soil fungal diversity in Yunnan province, PR China, a novel taxon, sp. nov., was identified based on combined morphological and molecular phylogenetic features. Morphologically, this species is characterized by having torulose, septate hyphae and swollen, terminal or intercalary conidiogenous cells arising at acute angles from aerial hyphae. Phylogenetic analysis of the combined sequences of the internal transcribed spacer, the small and large nuclear subunit of the rRNA gene and part of the β-tubulin gene confirmed the phylogenetic position of the new species within the genus .

Funding
This study was supported by the:
  • Innovative Research Group Project of the National Natural Science Foundation of China (Award 31970013)
    • Principle Award Recipient: Ze-FenYu
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005116
2021-11-30
2024-04-25
Loading full text...

Full text loading...

References

  1. de Hoog GS. Evolution of black yeasts: possible adaptation to the human host. Antonie van Leeuwenhoek 1993; 63:105–109 [View Article] [PubMed]
    [Google Scholar]
  2. Isola D, Zucconi L, Onofri S, Caneva G, de Hoog GS et al. Extremotolerant rock inhabiting black fungi from italian monumental sites. Fungal Divers 2015; 76:75
    [Google Scholar]
  3. De Hoog GS, Zeng JS, Harrak MJ, Sutton DA. Exophiala xenobiotica sp. nov., an opportunistic black yeast inhabiting environments rich in hydrocarbons. Antonie van Leeuwenhoek 2006; 90:257–268 [View Article] [PubMed]
    [Google Scholar]
  4. Carmichael JW. Cerebral mycetoma of trout due to a Phialophora-like fungus. Sabouraudia 1966; 5:120–123 [View Article] [PubMed]
    [Google Scholar]
  5. Borman AM, Fraser M, Szekely A, Larcombe DE, Johnson EM et al. Rapid identification of clinically relevant members of the genus Exophiala by matrix-assisted laser desorption ionization-time of flight mass spectrometry and description of two novel species, Exophiala campbellii and Exophiala lavatrina. J Clin Microbiol 2017; 55:1162–1176 [View Article] [PubMed]
    [Google Scholar]
  6. de Hoog GS, Vicente VA, Najafzadeh MJ, Harrak MJ, Badali H et al. Waterborne Exophiala species causing disease in cold-blooded animals. Persoonia 2011; 27:46–72 [View Article] [PubMed]
    [Google Scholar]
  7. Untereiner WA. Taxonomy of selected members of the ascomycete genus Capronia with notes on anamorph-teleomorph connections. Mycologia 1997; 89:120 [View Article]
    [Google Scholar]
  8. Sun W, Su L, Yang S, Sun J, Liu B et al. Unveiling the hidden diversity of rock-inhabiting fungi: Chaetothyriales from China. JoF 2020; 6:187 [View Article]
    [Google Scholar]
  9. Zeng JS, Sutton DA, Fothergill AW, Rinaldi MG, Harrak MJ et al. Spectrum of clinically relevant Exophiala species in the united states. J Clin Microbiol 2007; 45:3713–3720 [View Article] [PubMed]
    [Google Scholar]
  10. Addy HD, Piercey MM, Currah RS. Microfungal endophytes in roots. Can J Bot 2005; 83:1–13
    [Google Scholar]
  11. Maciá-Vicente JG, Glynou K, Piepenbring M. A new species of Exophiala associated with roots. Mycol Progress 2016; 15:18
    [Google Scholar]
  12. Iwatsu T, Udagawa S, Takase T. A new species of Exophiala recovered from drinking water. Mycotaxon 1991; 41:322–328
    [Google Scholar]
  13. Bates ST, Reddy GSN, Garcia-Pichel F. Exophiala crusticola anam. nov. (affinity Herpotrichiellaceae), a novel black yeast from biological soil crusts in the Western United States. Int J Syst Evol Microbiol 2006; 56:2697–2702 [View Article] [PubMed]
    [Google Scholar]
  14. Index Fungorum Index Fungorum; 2021 http://www.indexfungorum.org/names/Names.asp
  15. Haase G, Sonntag L, Melzer-Krick B, De Hoog GS. Phylogenetic inference by SSU-gene analysis of members of the Herpotrichiellaceae with special reference to human pathogenic species. Stud Mycol 1999; 43:80–97
    [Google Scholar]
  16. de Hoog GS, Vicente V, Caligiorne RB, Kantarcioglu S, Tintelnot K et al. Species diversity and polymorphism in the Exophiala spinifera clade containing opportunistic black yeast-like fungi. J Clin Microbiol 2003; 41:4767–4778 [View Article] [PubMed]
    [Google Scholar]
  17. Zheng H, Yu Z, Xu J, Castañeda-Ruiz RF, Qiao M. Ramichloridium endophyticum sp. nov., a novel species of endophytic fungus from Potamogeton pectinatus. Int J Syst Evol Microbiol 2020; 70:3433–3439 [View Article] [PubMed]
    [Google Scholar]
  18. Qiao M, Zheng H, Lv R, Yu Z. Neodactylariales, Neodactylariaceae (Dothideomycetes, Ascomycota): new order and family, with a new species from China. MycoKeys 2020; 73:69–85 [View Article] [PubMed]
    [Google Scholar]
  19. Zheng H, Li J, Guo JS, Qiao M, Yu ZF. Anacraspedodidymum submersum, a new species of aquatic hyphomycetes from southwest China. Int J Syst Evol Microbiol 2021; 71:004650
    [Google Scholar]
  20. Qiao M, Zhang Z, Yang L, Yu Z. Staphylotrichum sinense sp. nov., a new hyphomycete (Chaetomiaceae) from China. Int J Syst Evol Microbiol 2019; 71:004747 [View Article]
    [Google Scholar]
  21. Zheng H, Qiao M, Lv YF, Du X, Zhang KQ et al. New species of soil-inhabiting and aquatic endophytic Trichoderma from southwest China. Journal of Fungi 2021; 7:467
    [Google Scholar]
  22. Marizeth G, Lorenzo L, Michel DV, Giraldo LA et al. Diversity of yeast species from Dutch garden soil and the description of six novel Ascomycetes. FEMS Yeast Res 2018; 7:7
    [Google Scholar]
  23. Turner D, Kovacs W, Kuhls K, Lieckfeldt E, Peter B et al. Biogeography and phenotypic variation in Trichoderma sect. Longibrachiatum and associated Hypocrea species. Mycological Research 1997; 101:449–459
    [Google Scholar]
  24. White TJ, Bruns T, Lee S, Taylor J. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications vol. 18 1990 pp 315–322
    [Google Scholar]
  25. Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 1990; 172:4238–4246 [View Article] [PubMed]
    [Google Scholar]
  26. Rehner SA, Samuels GJ. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycological Res 1994; 98:625–634 [View Article]
    [Google Scholar]
  27. Glass NL, Donaldson GC. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Appl Environ Microbiol 1995; 61:1323–1330 [View Article] [PubMed]
    [Google Scholar]
  28. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 1997; 25:4876–4882 [View Article] [PubMed]
    [Google Scholar]
  29. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 1999; 41:95–98
    [Google Scholar]
  30. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874
    [Google Scholar]
  31. Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics 2001; 17:754–755 [View Article] [PubMed]
    [Google Scholar]
  32. Nylander JAA. MrModeltest v2.3 Evolutionary Biology Centre, Uppsala University; 2008 http://www.abc.se/~nylander/mrmodeltest2/mrmodeltest2.html/2008
  33. Sun X, Guo LD. Micronematobotrys, a new genus and its phylogenetic placement based on rDNA sequence analyses. Mycol Progress 2010; 9:567–574
    [Google Scholar]
  34. Rambaut A. FigTree v1.4.2 Edinburgh: Institute of Evolutionary Biology, University of Edinburgh; 2012 http://tree.bio.ed.ac.uk/software/ figtree/
  35. McGinnis MR. Taxonomy of Exophiala jeanselmei (Langeron) McGinnis and Padhye. Mycopathologia 1978; 65:79–87 [View Article] [PubMed]
    [Google Scholar]
  36. Zeng JS, De hoog GS. Exophiala spinifera and its allies: diagnostics from morphology to DNA barcoding. Med Mycol 2008; 46:193–208 46-3-193 [View Article]
    [Google Scholar]
  37. Sudduth EJ, Crumbley AJ 3rd, Farrar WE. Phaeohyphomycosis due to Exophiala species: clinical spectrum of disease in humans. Clin Infect Dis 1992; 15:639–644 [View Article] [PubMed]
    [Google Scholar]
  38. de Hoog GS, Takeo K, Yoshida S, Göttlich E, Nishimura K et al. Pleoanamorphic life cycle of Exophiala (Wangiella) dermatitidis. Antonie van Leeuwenhoek 1994; 65:143–153 [View Article] [PubMed]
    [Google Scholar]
  39. Uijthof JMJ. Relationships within the black yeast genus Exophiala based on ITS1 sequences. Mycological Research 1996; 100:1265–1271 [View Article]
    [Google Scholar]
  40. De Hoog GS, Mcginnis MR. Ascomycetous black yeasts. Stud Mycol 1987; 30:187–199
    [Google Scholar]
  41. Yong LK, Wiederhold NP, Sutton DA, Sandoval-Denis M, Lindner JR et al. Morphological and molecular characterization of Exophiala polymorpha sp. nov. isolated from sporotrichoid lymphocutaneous lesions in a patient with Myasthenia Gravis. J Clin Microbiol 2015; 53:2816–2822 [View Article] [PubMed]
    [Google Scholar]
  42. Arzanlou M, Groenewald JZ, Gams W, Braun U, Shin H-D et al. Phylogenetic and morphotaxonomic revision of Ramichloridium and allied genera. Stud Mycol 2007; 58:57–93 [View Article] [PubMed]
    [Google Scholar]
  43. Crous PW, Cowan DA, Maggs-Kölling G, Yilmaz N, Larsson E et al. Fungal planet description sheets: 1112-1181. Persoonia 2020; 45:251–409 [View Article] [PubMed]
    [Google Scholar]
  44. Vitale RG, Hoog G de. Molecular diversity, new species and antifungal susceptibilities in the Exophiala spinifera clade. Med Mycol 2002; 40:545–556
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005116
Loading
/content/journal/ijsem/10.1099/ijsem.0.005116
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error