1887

Abstract

A novel actinomycete, designated strain ASG 168, was isolated from cave rock collected from Stegodon Sea Cave in Thailand. Long chains of non-motile spores that were oval or spherical in shape with a smooth surface developed on aerial mycelia. Substrate mycelia fragmented into irregular rod-shaped elements. A polyphasic taxonomic study showed that strain ASG 168 had typical characteristics of members of the genus . 16S rRNA gene sequence analysis indicated that strain ASG 168 shared 97.5 % similarity with MS498 and 96.7 % with SCSIO 11529. Average nucleotide identity values with SCSIO 11529 and MS498 were 82.98 and 76.08 %, respectively. The cell-wall peptidoglycan contained -diaminopimelic acid. The whole-cell sugars contained ribose, arabinose and galactose. The predominant menaquinone was MK-9(H). The predominant fatty acids were iso-C, C and summed feature 3 (C 7/C 6). The phospholipid profile consisted of phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and two unknown phospholipids. The G+C content of the genomic DNA was 70.6 mol%. Differentiation of strain ASG 168 from closely related species was evident from digital DNA–DNA hybridization values of 29.2 and 21.3 % with and , respectively. Based on comparative analysis of phenotypic, chemotaxonomic and genotypic data, the novel actinomycete strain ASG 168 (=TBRC 13679=NBRC 114887) is proposed to be the type strain of a novel species, sp. nov.

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2021-11-22
2024-03-29
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