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Abstract

A light pink coloured bacterium, designated strain BN140002, was isolated from a soil sample collected in Goesan-gun, Chungcheongbuk-do, Republic of Korea. Cells of strain BN140002 were Gram-stain-negative, aerobic, motile and rod-shaped. Phylogenetic analysis based on 16S rRNA gene sequences showed 94.7, 94.7, 93.9, 93.3, 93.4 and 93.0% similarities to KCTC 22346, DSM 22962, HL-109, R491, EBR-4-1 and PC-2, respectively. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine. The major cellular fatty acids were summed feature 8 (C 7 and/or C 6c) and summed feature 1 (C aldehyde and/or unknown 10.98) and the predominant ubiquinone was Q-10. The genomic DNA G+C content of strain BN140002 was 70.1 mol%. The genomic orthoANI values between strain BN140002 and KCTC 22346 and DSM 22962 were 75.0 and 74.8 %, respectively. Strain BN140002 had a class I-C type CRISPR-Cas system (CRISPR-associated helicase Cas3, CRISPR-associated protein Cas8c, CRISPR-associated protein Cas7, CRISPR-associated RecB family exonuclease Cas4, CRISPR-associated protein 1, 2). Based on phenotypic, chemotaxonomic and phylogenetic data, strain BN140002 should be assigned as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BN140002 (=KCTC 42643=CCTCC AB 2017173).

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2022-02-28
2022-05-18
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