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Abstract

A novel actinobacterium, designated strain SYSU M44304, was isolated from freshwater samples in the Pearl River Estuary. The isolate was Gram-stain-positive, aerobic, coccus-shaped, oxidase-positive and motile. The cell wall contained -diaminopimelic acid as its diagnostic diamino acid. The predominant menaquinone was MK-8. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine and seven unidentified phospholipids. The major fatty acids were C and C. The G+C content based on genomic DNA was 73.2 mol %. The nearest phylogenetic neighbours to the novel strain were NBRC 104925 and YIM 101593. On the basis of chemotaxonomic and physiological characteristics and phylogenetic analysis, strain SYSU M44304 should be considered to represent a novel species of a new genus in the family , for which we propose the name gen. nov., sp. nov. The type strain of is SYSU M44304 (=NBRC 114808=CGMCC 1.18608).

Funding
This study was supported by the:
  • Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (Award 311021004)
    • Principle Award Recipient: Jia-LingLi
  • Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (Award 311021006)
    • Principle Award Recipient: Wen-JunLi
  • guangdong basic and applied basic research foundation, china (Award 2020A1515011139)
    • Principle Award Recipient: Jia-LingLi
  • postdoctoral research foundation of china (Award 2018M643294)
    • Principle Award Recipient: Jia-LingLi
  • national natural science foundation of china (Award 31850410475)
    • Principle Award Recipient: Jia-LingLi
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