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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 11

sp. nov. isolated from trachea of , sp. nov. and sp. nov. from human faeces No Access

Abstract

Six novel facultatively anaerobic, Gram-stain-positive, rod-shaped, non-haemolytic bacteria (zg-320/zg-336, zg-917/zg-910 and zg-913/zg-915) isolated from animal tissues and human faeces were found to belong to the genus based on the phylogenetic analyses of 16S rRNA gene and 262 core genes set. Based on the greatest degree of 16S rRNA similarity, zg-320/zg-336 had the highest 16S rRNA gene similarity to DSM 44353 (97.51 %), zg-917/zg-910 to DSM 44184 (98.68 %), and zg-913/zg-915 to subsp. CIP 103500 (98.79 %). The three novel type strains had a relatively high DNA G+C content (61.2–64.4 mol%), low DNA relatedness and ANI values with their respective neighbours: 23.5/72.7 %, 25.0/72.3%and 22.6/73.1 % (zg-320 vs. CIP 106629, DSM 45100 and DSM 45110); 24.4/82.3% and 23.7/81.3 % (zg-917 vs. DSM 44184 and JCB); 26.8/83.7% and 27.7/84.4 % (zg-913 vs. ATCC 700355 and subsp. CCUG 32105). The three novel species had C, C, C 9 and C ante/C 6,9 as the major cellular fatty acids; MK-8(H) in strain zg-917 and MK-9(H) in strains zg-320 and zg-913 were found to be the major respiratory quinones. For the three novel species, the detected major polar lipids included diphosphatidylglycerol, phosphatidyl inositol mannoside, phosphatidylglycerol and phosphatidylinositol, the cell-wall peptidoglycan was based on -DAP, and the whole-cell sugars mainly included ribose, arabinose and galactose. The three novel species grew optimally at 35–37 °C, 0.5 % (w/v) NaCl and pH 7.0–8.0; notably, they were tolerant of 10.5 % (w/v) NaCl. Based on the results of these comprehensive analyses, three novel species in the genus are proposed, aptly named sp. nov. (zg-320 = GDMCC 1.1719 = JCM 34106), sp. nov. (zg-917 = GDMCC 1.1707 = JCM 34094) and sp. nov. (zg-913 = GDMCC 1.1706 = JCM 34398).

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  • Accepted:
  • Published Online:
Keyword(s): ANI , Corynebacterium , dDDH , novel species and phylogenetic analysis
© 2021 The Authors
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/content/journal/ijsem/10.1099/ijsem.0.005069
2021-11-30
2024-04-25
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Corynebacterium zhongnanshanii sp. nov. isolated from trachea of Marmota himalayana, Corynebacterium lujinxingii sp. nov. and Corynebacterium wankanglinii sp. nov. from human faeces
Int J Syst Evol Microbiol 71, 005069 (2021); https://doi.org/10.1099/ijsem.0.005069
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/content/journal/ijsem/10.1099/ijsem.0.005069
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