@article{mbs:/content/journal/ijsem/10.1099/ijsem.0.005054, author = "Booncharoen, Auttaporn and Visessanguan, Wonnop and Kuncharoen, Nattakorn and Yiamsombut, Supalurk and Santiyanont, Pannita and Mhuantong, Wuttichai and Rojsitthisak, Pornchai and Tanasupawat, Somboon", title = "Halobacillus fulvus sp. nov., a moderately halophilic bacterium isolated from shrimp paste (Ka-pi) in Thailand", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2021", volume = "71", number = "11", pages = "", doi = "https://doi.org/10.1099/ijsem.0.005054", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005054", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "Ka-pi", keywords = "Halobacillus fulvus", keywords = "moderate halophile", keywords = "shrimp paste", eid = "005054", abstract = "An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6T, was isolated from shrimp paste (Ka-pi) collected from Samut Sakhon Province, Thailand. Phylogenetic analysis revealed that strain SKP4-6T belonged to the genus Halobacillus and was most closely related to Halobacillus salinus JCM 11546T (98.6 %), Halobacillus locisalis KCTC 3788T (98.6 %) and Halobacillus yeomjeoni KCTC 3957T (98.6 %) based on 16S rRNA gene sequence similarity. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKP4-6T and its related species were 18.2–19.3 % and 69.84–84.51 %, respectively, which were lower than the threshold recommended for species delineation. The strain grew optimally at 30–40 °C, at pH 7.0 and with 10–15 % (w/v) NaCl. It contained l-Orn–d-Asp in the cell wall peptidoglycan. The DNA G+C content was 44.8 mol%. The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The predominant isoprenoid quinone was MK-7. Phosphatidylglycerol and diphosphatidylglycerol were present as major polar lipids. Based on this polyphasic approach, digital DNA–DNA relatedness and ANI values, strain SKP4-6T represents a novel species of the genus Halobacillus , for which the name Halobacillus fulvus sp. nov. is proposed. The type strain is SKP4-6T (=JCM 32624T=TISTR 2595T).", }