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Abstract

An aerobic, Gram-stain-positive, endospore-forming, rod-shaped and moderately halophilic strain SKP4-6, was isolated from shrimp paste () collected from Samut Sakhon Province, Thailand. Phylogenetic analysis revealed that strain SKP4-6 belonged to the genus and was most closely related to JCM 11546 (98.6 %), KCTC 3788 (98.6 %) and KCTC 3957 (98.6 %) based on 16S rRNA gene sequence similarity. The digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain SKP4-6 and its related species were 18.2–19.3 % and 69.84–84.51 %, respectively, which were lower than the threshold recommended for species delineation. The strain grew optimally at 30–40 °C, at pH 7.0 and with 10–15 % (w/v) NaCl. It contained -Orn-Asp in the cell wall peptidoglycan. The DNA G+C content was 44.8 mol%. The major fatty acids were iso-C, anteiso-C and anteiso-C. The predominant isoprenoid quinone was MK-7. Phosphatidylglycerol and diphosphatidylglycerol were present as major polar lipids. Based on this polyphasic approach, digital DNA–DNA relatedness and ANI values, strain SKP4-6 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SKP4-6 (=JCM 32624=TISTR 2595).

Funding
This study was supported by the:
  • National Research Council of Thailand (Award GB-A_60_019_33_04)
    • Principle Award Recipient: SomboonTanasupawat
  • Thailand Graduate Institute of Science and Technology, TGIST (Award TG-22-09-58-036D)
    • Principle Award Recipient: AuttapornBooncharoen
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/content/journal/ijsem/10.1099/ijsem.0.005054
2021-11-26
2022-01-28
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