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Abstract

This paper presents a polyphasic taxonomic study of a Gram-stain-negative bacterium designated GA093, a soil isolate capable of benzo(α)pyrene degradation. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain GA093 is a member of the genus , and formed an independent phylogenetic line while clustering with the type strains of , and . Strain GA093 was facultatively anaerobic, and could grow at 4–33 °C (optimum, 30 °C), at pH 6–11 (optimum, pH 7) and in the presence of 0–2 % (w/v) NaCl (optimum, 0 %). Strain GA093 was capable of producing acid from various carbon sources, which was comparable to other related species of . The strain contained MK-6 as the only isoprenoid quinone, iso-C as the major cellular fatty acid, phosphatidylethanolamine and phosphatidylinositol as diagnostic polar lipids, and -homospermidine as the major polyamine. The chemotaxonomic properties of strain GA093 were consistent with the general properties of except the presence of phosphatidylinositol, which distinguished it from other related species. The total stretch of the obtained genome of GA093 was 5.05 Mbp, and the DNA G+C content was 34.79 mol%. The genome contained genes potentially related to the degradation of aromatic hydrocarbons. On the basis of the present polyphasic analysis, strain GA093 was found to have properties that distunguished it as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GA093 (=KCTC 72594=LMG 31760).

Funding
This study was supported by the:
  • National Institute of Biological Resources
    • Principle Award Recipient: SeungBum Kim
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2021-10-20
2024-07-13
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