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Volume 71, Issue 10

sp. nov., a commensal spirochaete isolated from human faeces Open Access

Abstract

A Gram-stain-negative, obligatory anaerobic spirochaete (RCC2812) was isolated from a faecal sample obtained from an individual residing in a remote Amazonian community in Peru. The bacterium showed highest 16S rRNA gene sequence similarity to the pig intestinal spirochete (89.48 %). Average nucleotide identity values between strain RCC2812 and all available genomes from validated type strains were all <73 %, thus clearly lower than the species delineation threshold. The DNA G+C content of RCC2812 was 41.24 mol%. Phenotypic characterization using the API-ZYM and API 20A systems confirmed the divergent position of this bacterium within the genus . Strain RCC2812 could be differentiated from the phylogenetically most closely related by the presence of alkaline phosphatase and α -glucosidase activities. Unlike , strain RCC2812 grew equally well with or without serum. Strain RCC2812 is the first commensal isolated from the human faecal microbiota of remote populations, and based on the collected data represents a novel species for which the name sp. nov. is proposed. The type strain is RCC2812 (=LMG 31794=CIP 111910).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): faeces , gut , human , microbiome , spirochaete and Treponema
Funding
This study was supported by the:
  • vlaams instituut voor biotechnologie
    • Principle Award Recipient: JeroenRaes
  • fonds wetenschappelijk onderzoek (Award 1221620N)
    • Principle Award Recipient: RodrigoBacigalupe
  • fonds wetenschappelijk onderzoek (Award 1234321N)
    • Principle Award Recipient: RaulTito Tadeo
  • fonds wetenschappelijk onderzoek (Award 11ZF416N)
    • Principle Award Recipient: ClaireBelkhou
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License.
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2021-10-21
2024-04-24
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Treponema peruense sp. nov., a commensal spirochaete isolated from human faeces
Int J Syst Evol Microbiol 71, 005050 (2021); https://doi.org/10.1099/ijsem.0.005050
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