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Volume 71, Issue 10

gen. nov., sp. nov., a novel bacterium of the family isolated from a shrimp pond No Access

Abstract

A Gram-stain-negative, non-motile, rod-shaped, aerobic bacterium (designated as LMIT005) was isolated from shrimp ponds in Shantou, China. The new isolate was characterized taxonomically using a polyphasic approach. Based on 16S rRNA gene sequence analysis, strain LMIT005 was found to be affiliated with the family of the order while appearing as a distinct lineage. The 16S rRNA gene sequence similarity between strain LMIT005 and KCTC 12309, the closest type strain in the family, was 91.3 %. Strain LMIT005 grew optimally at 25 °C, pH 7 and in the presence of 2.0 % (w/v) NaCl. The DNA G+C content (data from genome sequence) was 40.5 mol%. Compared with reference strain KCTC 12309, the average nucleotide identity (ANI) of LMIT005 was 70 %. The sole respiratory quinone of LMIT005 was menaquinone (MK-7), and the major fatty acids were summed feature 3 (C ω6c / C ω7c). The polar lipids of strain LMIT005 were mainly composed of phosphatidylethanolamine, phosphatidylcholine, two unidentified amino lipids, two unidentified lipids, one unidentified glycolipid and one unidentified phospholipid. The draft genome of strain LMIT005 comprised 3 089 781 bp (3.09 Mb) nucleotides and 2773 genes. Antimicrobial resistant-related genes ( and ) were annotated in the genome of strain LMIT005, which indicated that it might be able to resist β-lactam antibiotics. This was further verified by antimicrobial resistant test. Given its distinct genomic, morphological, and physiological differences from previously described type strains, strain LMIT005 is proposed as a representative of a novel genus of the family with the name gen. nov., sp. nov. The type strain is LMIT005 (=KCTC 82616=CICC 25047).

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Keyword(s): antimicrobial resistant genes , average amino acid identity , Cyclobacteriaceae , percentage of conserved proteins and xenobiotics biodegradation
Funding
This study was supported by the:
  • guangdong science and technology department (Award 2019ST023)
    • Principle Award Recipient: HuiWang
  • national natural science foundation of china (Award 92051118)
    • Principle Award Recipient: HuiWang
  • key special project for introduced talents team of southern marine science and engineering guangdong laboratory (guangzhou) (Award GML2019ZD0606)
    • Principle Award Recipient: HuiWang
© 2021 The Authors
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2021-10-08
2024-04-19
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Penaeicola halotolerans gen. nov., sp. nov., a novel bacterium of the family Cyclobacteriaceae isolated from a shrimp pond
Int J Syst Evol Microbiol 71, 005047 (2021); https://doi.org/10.1099/ijsem.0.005047
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