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Abstract

A bacterial strain designated 32A was isolated from the skin of an Anderson’s salamander () and subjected to a comprehensive taxonomic study. The strain was Gram-stain-negative, rod-shaped, non-motile, oxidase- and urease-negative, and catalase-positive. 16S rRNA gene sequence comparisons placed the strain in the genus with highest sequence similarities to A4T-83 (95.2%), CB-286403 (95.1%) and E100 (94.9%). Genomic sequence analysis revealed a size of 5.3 Mbp, a G+C-content of 62.2 mol% and highest ANI values with (71.2%), (71.4%) and (69.5%). In the polyamine pattern, 1,3-diaminopropane and spermidine were predominant. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. The quinone system was composed of the major menaquinones MK-9 and MK-10. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, the unidentified aminolipid AL2, the unidentified phospholipid PL2 and the unidentified aminophospholipid APL1. The fatty acid profile contained major amounts of iso-C, iso-C, C and C ω9. In addition, C, C anteiso-C, summed feature 2 (C 3OH and/or iso-C I), and the hydroxylated fatty acids iso-C 3OH, iso-C 3OH and C 3-OH were detected. Physiologically, strain 32A is distinguishable from its next relatives. Based on phylogenetic, genomic, physiological and chemotaxonomic data, strain 32A represents a novel species of the genus for which we propose the name sp. nov. The type strain is 32A (=CCM 9141=LMG 32214).

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2021-10-08
2021-10-26
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