1887

Abstract

A bacterial strain, designated TCH3-2, was isolated from the rhizosphere of tomato plant grown at Dong-A University Agricultural Experiment Station, Republic of Korea. The strain was Gram-stain-negative, obligate aerobic, orange yellow-coloured, motile by gliding and short rod-shaped. Strain TCH3-2 only grew on 1/2 tryptic soy agar and Luria–Bertani agar among the media tested, with optimum growth at 28 °C and pH 7. Salt of 1 % NaCl was necessary to support the growth of TCH3-2. Strain TCH3-2 produced flexirubin-type pigments. The predominant cellular fatty acids were iso-C (55.6 %), iso-C 3-OH (17.9 %), summed feature 9 (comprising C 10-methyl and/or iso-C 9; 10.5 %), iso-C 3-OH (4.8 %) and anteiso-C (2.3 %). The major menaquinone was menaquinone-6 and the major polar lipids were phosphatidylethanolamine, five unknown aminolipids and three unknown lipids. Phylogenetic analysis based on 16S rRNA sequences indicated that TCH3-2 was closely related to DS-12 (95.16 %), SW105 (95.14 %) and YIM 102796 (94.54 %). The draft genome of TCH3-2 comprised . 2.8 Mb with a G+C content of 34.61 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between TCH3-2 and closely related species showed that it belongs to a distinct species. Furthermore, the results of morphological, physiological and biochemical tests allowed further phenotypic differentiation of TCH3-2 from its closest relatives. Thus, chemotaxonomic characteristics together with phylogenetic affiliation illustrate that TCH3-2 represents a novel species of the genus , for which the name sp. nov. (type strain TCH3-2=KACC 19054=JCM 34025) is proposed.

Funding
This study was supported by the:
  • Rural Development Administration (Award PJ01313101)
    • Principle Award Recipient: LeeSeon-Woo
  • National Research Foundation of Korea (Award 2020R1A2C3005453 and 2020R1A6A1A03047729)
    • Principle Award Recipient: LeeSeon-Woo
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2021-10-01
2021-10-23
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