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Volume 71, Issue 9

sp. nov. and sp. nov., two novel actinomycetes isolated from the deep Southern Ocean Open Access

Abstract

The actinomycetes strains KRD168 and KRD185 were isolated from sediments collected from the deep Southern Ocean and, in this work, they are described as representing two novel species of the genus through a polyphasic approach. Despite sharing >99 % 16S rRNA gene sequence similarity with other members of the genus, comparative genomic analysis allowed species delimitation based on average nucleotide identity and digital DNA–DNA hybridization. The KRD168 genome is characterized by a size of 6.31 Mbp and a G+C content of 73.44 mol%, while the KRD185 genome has a size of 6.82 Mbp and a G+C content of 73.98 mol%. Both strains contain -diaminopimelic acid as the diagnostic diamino acid, glucose as the major whole-cell sugar, MK-8(H) as a major menaquinone and -branched hexadecanoic acid as a major fatty acid. Biochemical and fatty acid analyses also revealed differences between these strains and their phylogenetic neighbours, supporting their status as distinct species. The names sp. nov. (type strain KRD168=DSM 111918=NCIMB 15270) and (type strain KRD185=DSM 111919=NCIMB 15269) are proposed.

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  • Accepted:
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Keyword(s): Actinobacteria , ocean , Pseudonocardia and sediments
Funding
This study was supported by the:
  • Programa de Innovación y Capital Humano para la Competitividad (PINN) of The Ministry of Science, Technology and Telecommunications of Costa Rica (MICITT) (Award 2-1-4-17-1-037.)
    • Principle Award Recipient: JonathanParra
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-09-28
2024-04-25
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Pseudonocardia abyssalis sp. nov. and Pseudonocardia oceani sp. nov., two novel actinomycetes isolated from the deep Southern Ocean
Int J Syst Evol Microbiol 71, 005032 (2021); https://doi.org/10.1099/ijsem.0.005032
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