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Abstract

A Gram-stain-positive, strictly aerobic, motile, endospore-forming, milk-white, indole acetic acid-producing, rod-shaped bacterial strain, designated as HU2P27, was isolated from a shoot of collected in Tumd Right Banner, Inner Mongolia, PR China. Strain grew at 10–40 °C (optimum, 30 °C), at pH 6.0–9.0 (optimum, pH 7.0) and with 0–14.0 % NaCl (optimum, 5.0–8.0 %). The strain tested positive for oxidase, catalase and nitrate reductase. The phylogenetic trees based on the 16S rRNA gene sequence and the core genome both showed that strain HU2P27 clustered with BH30097, sharing 97.7 % and <97.0 % of 16S rRNA gene similarity with BH30097 and any other type strain. Strain HU2P27 contained MK-7 as the major respiratory quinone. Its major fatty acids were anteiso-C and iso-C, and the major polar lipids were phosphatidylglycerol, diphosphatidylglycerol and four unidentified phospholipids. The genomic DNA G+C content was 36.0 mol%. The average nucleotide identity, amino acid identity and digital DNA–DNA hybridization values of strain HU2P27 with BH30097 were 71.7, 69.2 and 19.4%, respectively. The phylogenetic, physiological and phenotypic results allowed the discrimination of strain HU2P27 from its phylogenetic relatives. The name sp. nov. is therefore proposed. The type strain is strain HU2P27 (=CGMCC 1.18646=KCTC 43248). Based on the results of 16S rRNA gene and genome analyses, we propose the reclassification of Lee . 2012 as a later heterotypic synonym of Kim . 2011.

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31960020)
    • Principle Award Recipient: Ji-QuanSun
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2021-10-07
2021-10-24
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