1887

Abstract

A strictly anaerobic bacterium, strain PLL0, was isolated from petroleum-contaminated soil sampled in Gansu Province, PR China. Cells were rods, non-motile and Gram-stain-positive. The strain grew at 25–37 °C (optimum, 30 °C) in the presence of 0–3 % (w/v) NaCl (optimum, 2 %). Strain PLL0 was able to reduce ferrihydrite, Fe(III) citrate and thiosulphate. The 16S rRNA gene analysis revealed that this strain clustered with the genus , and showed highest similarity to UKTL (95.4 %) followed by Co23 (93.9 %). However, strains PLL0 and UKTL showed no more than 94.0 % similarity to other species of the genus , and formed an independent group in the phylogenetic tree. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain PLL0 and species (except for ) were 67.4–68.5 % and 12.6–12.7 %, respectively, which are far below the threshold for delineation of a new species. Based on ANI, dDDH, average amino acid identity, phylogenetic analysis and physiologic differences from the previously described taxa, we suggest that strain PLL0 represents a novel species of a novel genus, for which the name gen. nov. sp. nov. is proposed. The type strain is PLL0 (=MCCC 1K05549=KCTC 25248). We also propose the reclassification of as comb. nov.

Funding
This study was supported by the:
  • science and technology program of guangzhou (Award 202002030123)
    • Principle Award Recipient: GuiqinYang
  • natural science foundation of guangdong province (Award 2021A1515012570)
    • Principle Award Recipient: GuiqinYang
  • national natural science foundation of china (Award 42077211)
    • Principle Award Recipient: LiZhuang
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2021-09-24
2021-10-24
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