1887

Abstract

During a study of the bacterial diversity of mangrove habitats, a novel Gram-stain-negative, rod-shaped bacterium designated as SAOS 153D was isolated. Sequence alignment and molecular phylogenetic analyses based on 16S rRNA and core gene sequence of strain SAOS 153D with closely related taxa revealed a sequence identity of 99.4 % and clustering with DX5-10. The fatty acids summed feature 8 (C 7/C 6) and the lipids phosphatidylglycerol, phosphatidylethanolamine and an unknown phospholipid were the major components of the cell wall. The only ubiquinone type present was Q-10. The genomic DNA G+C content of the strain calculated from whole genome sequencing was 66.9 mol%. These chemotaxonomic and genomic characteristics supported the molecular phylogenetic analysis and placed the strain well within the radiation of the genus . The overall genome related indices using digital DNA–DNA hybridization (35.4 %) and ortho-average nucleotide identity (88.1 %) values were much lower than the recommended thresholds for species delineation, which further consolidated the novel species status of strain SAOS 153D within the genus as sp. nov. The type strain is SAOS 153D (=JCM 31345=KCTC 52280=MTCC 12749).

Keyword(s): 16S rRNA , FAME , phylogeny and SAOS 153DT
Funding
This study was supported by the:
  • Ministry of Earth Sciences (Award GAP0137)
    • Principle Award Recipient: SrinivasanKrishnamurthi
  • Department of Biotechnology, Ministry of Science and Technology (Award BT/PR7368/INF/22/177/2012)
    • Principle Award Recipient: KrishnamurthiSrinivasan
  • Council of Scientific and Industrial Research, India (Award BSC0402)
    • Principle Award Recipient: SrinivasanKrishnamurthi
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2021-09-22
2021-10-24
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