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Volume 71, Issue 9

sp. nov., isolated from a freshwater alga No Access

Abstract

A Gram-stain-negative, strictly aerobic, catalase-negative, oxidase-positive and non-motile rod-shaped bacterium, designated strain CrO1, was isolated from a freshwater alga in the Nakdong river of South Korea. Colonies of CrO1 were white, convex and circular and growth was observed at 25–40 °C (optimum, 37 °C) and pH 6.0–9.0 (optimum, pH 7) and in the presence of 0–0.5 % (w/v) NaCl (optimum, 0 %). CrO1 contained C, summed feature 5 (comprising Cante and/or Cω6,9), C, summed feature 3 (comprising Cω7 and/or Cω6) and summed feature 8 (comprising Cω7 and/or Cω6) as the major cellular fatty acids (>5 %) and ubiquinone-8 as the sole respiratory quinone. Phosphatidylethanolamine was detected as the major polar lipid. The DNA G+C content of CrO1, calculated from the whole genome sequence was 69.6 mol%. CrO1 was most closely related to 18x22-1 with a 97.6 % 16S rRNA sequence similarity and shared less than 97.4 % 16S rRNA sequence similarities with other type strains. Phylogenetic analyses based on the 16S rRNA gene and whole genome sequences revealed that CrO1 formed a distinct phyletic lineage within the genus . On the basis of the results of phenotypic, chemotaxonomic and molecular analysis, CrO1 clearly represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CrO1 (=KACC 19926=JCM 33302).

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Keyword(s): Betaproteobacteria , Cryptomonas obovoidea , freshwater alga , new taxa and Ramlibacter algicola
Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR No. 2020-02-001)
    • Principle Award Recipient: CheOk Jeon
  • National Research Foundation of Korea (Award 2018R1A5A1025077)
    • Principle Award Recipient: CheOk Jeon
© 2021 The Authors
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2021-09-22
2024-04-19
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Ramlibacter algicola sp. nov., isolated from a freshwater alga Cryptomonas obovoidea
Int J Syst Evol Microbiol 71, 005010 (2021); https://doi.org/10.1099/ijsem.0.005010
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