%0 Journal Article %A Li, Chenxu %A Cao, Peng %A Du, Chuanjiao %A Zhang, Xue %A Bing, Hui %A Li, Lei %A Sun, Peng %A Xiang, Wensheng %A Zhao, Junwei %A Wang, Xiangjing %T Massilia rhizosphaerae sp. nov., a rice-associated rhizobacterium with antibacterial activity against Ralstonia solanacearum %D 2021 %J International Journal of Systematic and Evolutionary Microbiology, %V 71 %N 9 %@ 1466-5034 %C 005009 %R https://doi.org/10.1099/ijsem.0.005009 %K Ralstonia solanacearum %K antibacterial activity %K whole genome analysis %K polyphasic approach %K Massilia rhizosphaerae sp. nov. %I Microbiology Society, %X A novel rhizobacterium, designated strain NEAU-GH312T, with antibacterial activity against Ralstonia solanacearum was isolated from rhizosphere soil of rice (Heilongjiang Province, PR China) and characterized with a polyphasic approach. Cells of strain NEAU-GH312T were Gram-stain-negative, aerobic, non-spore-forming, motile with peritrichous flagella and rod-shaped. Colonies were light orange, convex and semi-translucent on Reasoner's 2A (R2A) agar after 2 days of incubation at 28 °C. Growth was observed on R2A agar at 10–40 °C, pH 4.0–8.0 and with 0–5 % (w/v) NaCl. The respiratory quinone was ubiquinone Q-8. The major cellular fatty acids of strain NEAU-GH312T were C16 : 1  ω7c and/or C16 : 1  ω6c, C16 : 0 and C18 : 1  ω7c and/or C18 : 1  ω6c. The main polar lipids were phosphatidylglycerol, phosphatidylethanolamine and diphosphatidylglycerol. Phylogenetic analyses confirmed the well-supported affiliation of strain NEAU-GH312T within the genus Massilia , close to the type strains of Massilia arvi THG-RS2OT (98.7 %), Massilia norwichensis NS9T (98.7 %) and Massilia kyonggiensis TSA1T (98.6 %). Strain NEAU-GH312T had a genome size of 6.68 Mb and an average DNA G+C content of 66.3 mol%. Based on the genotypic, phenotypic and chemotaxonomic data obtained in this study, strain NEAU-GH312T could be classified as representative of a novel species of the genus Massilia , for which the name Massilia rhizosphaerae sp. nov. is proposed, with strain NEAU-GH312T (=DSM 109722T=CCTCC AB 2019142T) as the type strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.005009