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Abstract

An obligately anaerobic, non-motile, Gram-negative and rod-shaped strain (AGMB03916) was isolated from faeces of a 2-week-old piglet raised at the National Institute of Animal Science in Wanju, Republic of Korea. Growth of strain AGMB03916 occurred at 30–45 °C (optimum, 37 °C), at pH 6–9 (optimum, pH 8) and in the presence of 0.5–1.0 % (w/v) NaCl. Based on the results of 16S rRNA gene sequence analyses, strain AGMB03916 was closely related to two validly published species of the genus , and . The 16S rRNA gene sequence similarity of strain AGMB03916 compared to M12 (=KCTC 5793) and M16 (=KCTC 5443) were 96.3 and 95.0 %, respectively. The genomic DNA G+C content of strain AGMB03916 was 46.4 mol%. The average nucleotide identity values between strain AGMB03916 and the reference strains were 74.9–78.5 %. Cells were able to utilize -glucose, lactose, sucrose, maltose, salicin, aesculin hydrolysis, cellobiose and raffinose. The major end product of metabolism was acetate. The major cellular fatty acids (>10 %) of the isolate were iso-C, anteiso-C, C, C 3-OH and summed feature 11 (iso-C 3-OH and/or C DMA). On the basis of the genotypic, biochemical, chemotaxonomic, phenotypic and phylogenetic data, strain AGMB03916 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AGMB03916 (=KCTC 25014=GDMCC 1.2574).

Funding
This study was supported by the:
  • national research foundation of korea (Award 2016M3A9F3946674)
    • Principle Award Recipient: Jung-SookLee
  • korea evaluation institute of industrial technology (Award 20009412)
    • Principle Award Recipient: Seung-HwanPark
  • national research foundation of korea (Award 2019M3A9F3065226)
    • Principle Award Recipient: NotApplicable
  • korea research institute of bioscience and biotechnology (Award KGM5232113)
    • Principle Award Recipient: NotApplicable
  • national research foundation of korea (Award NRF-2021M3H9A1030164)
    • Principle Award Recipient: NotApplicable
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2021-09-21
2021-10-24
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