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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 9

sp. nov., isolated from porcine faeces Free

Abstract

An obligately anaerobic, non-motile, Gram-negative and rod-shaped strain (AGMB03916) was isolated from faeces of a 2-week-old piglet raised at the National Institute of Animal Science in Wanju, Republic of Korea. Growth of strain AGMB03916 occurred at 30–45 °C (optimum, 37 °C), at pH 6–9 (optimum, pH 8) and in the presence of 0.5–1.0 % (w/v) NaCl. Based on the results of 16S rRNA gene sequence analyses, strain AGMB03916 was closely related to two validly published species of the genus , and . The 16S rRNA gene sequence similarity of strain AGMB03916 compared to M12 (=KCTC 5793) and M16 (=KCTC 5443) were 96.3 and 95.0 %, respectively. The genomic DNA G+C content of strain AGMB03916 was 46.4 mol%. The average nucleotide identity values between strain AGMB03916 and the reference strains were 74.9–78.5 %. Cells were able to utilize -glucose, lactose, sucrose, maltose, salicin, aesculin hydrolysis, cellobiose and raffinose. The major end product of metabolism was acetate. The major cellular fatty acids (>10 %) of the isolate were iso-C, anteiso-C, C, C 3-OH and summed feature 11 (iso-C 3-OH and/or C DMA). On the basis of the genotypic, biochemical, chemotaxonomic, phenotypic and phylogenetic data, strain AGMB03916 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is AGMB03916 (=KCTC 25014=GDMCC 1.2574).

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Keyword(s): Bacteroides , Obligate anaerobe , Phocaeicola faecicola and Porcine faeces
Funding
This study was supported by the:
  • national research foundation of korea (Award 2016M3A9F3946674)
    • Principle Award Recipient: Jung-SookLee
  • korea evaluation institute of industrial technology (Award 20009412)
    • Principle Award Recipient: Seung-HwanPark
  • national research foundation of korea (Award 2019M3A9F3065226)
    • Principle Award Recipient: NotApplicable
  • korea research institute of bioscience and biotechnology (Award KGM5232113)
    • Principle Award Recipient: NotApplicable
  • national research foundation of korea (Award NRF-2021M3H9A1030164)
    • Principle Award Recipient: NotApplicable
© 2021 The Authors
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2021-09-21
2025-01-17
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Phocaeicola faecicola sp. nov., isolated from porcine faeces
Int J Syst Evol Microbiol 71, 005008 (2021); https://doi.org/10.1099/ijsem.0.005008
/content/journal/ijsem/10.1099/ijsem.0.005008
/content/journal/ijsem/10.1099/ijsem.0.005008
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