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Abstract

A strictly anaerobic bacterial strain (27-44) was isolated from a stool specimen from an autistic child collected in PR China. The strain was Gram-stain-positive, non-motile, non-pigmented, non-spore-forming, and cells were oval to rod-shaped. Strain 27-44 grew at 20–40 °C (optimal at 37 °C) and at pH 6.0–10 (optimal at 6.0–8.0). The major polar lipids were one phospholipid, two glycolipids, two aminophospholipids and one unidentified lipid. The major cellular fatty acids of strain 27-44 were C and C 2-OH. The end product of glucose fermentation was mainly butyric acid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 27-44 was a member of the genus and phylogenetically closely related to ATCC 29174 (with 97.8 % seque nce similarity). The genome of strain 27-44 was 3.5 Mbp with a DNA G+C content of 42.36 mol%. A total of 3436 genes were predicted and, of these, 3133 genes were annotated by KEGG. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons, strain 27-44 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 27-44= CGMCC 1.5285=NBRC 113774.

Funding
This study was supported by the:
  • National Nature Science Foundation of China (Award 31670102)
    • Principle Award Recipient: Yu-JingWang
  • Strategic Priority CAS Project (Award XDB38020000)
    • Principle Award Recipient: Yu-JingWang
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2021-09-21
2021-10-24
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