1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 9

sp. nov., isolated from human feces No Access

Abstract

A strictly anaerobic bacterial strain (27-44) was isolated from a stool specimen from an autistic child collected in PR China. The strain was Gram-stain-positive, non-motile, non-pigmented, non-spore-forming, and cells were oval to rod-shaped. Strain 27-44 grew at 20–40 °C (optimal at 37 °C) and at pH 6.0–10 (optimal at 6.0–8.0). The major polar lipids were one phospholipid, two glycolipids, two aminophospholipids and one unidentified lipid. The major cellular fatty acids of strain 27-44 were C and C 2-OH. The end product of glucose fermentation was mainly butyric acid. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 27-44 was a member of the genus and phylogenetically closely related to ATCC 29174 (with 97.8 % seque nce similarity). The genome of strain 27-44 was 3.5 Mbp with a DNA G+C content of 42.36 mol%. A total of 3436 genes were predicted and, of these, 3133 genes were annotated by KEGG. On the basis of phenotypic, chemotaxonomic and phylogenetic comparisons, strain 27-44 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is 27-44= CGMCC 1.5285=NBRC 113774.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Blautia intestinalis , faeces , human intestinal microbiota and strain 27-44T
Funding
This study was supported by the:
  • National Nature Science Foundation of China (Award 31670102)
    • Principle Award Recipient: Yu-JingWang
  • Strategic Priority CAS Project (Award XDB38020000)
    • Principle Award Recipient: Yu-JingWang
© 2021 The Authors
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.005005
2021-09-21
2024-04-25
Loading full text...

Full text loading...

References

  1. Lozupone CA, Stombaugh JI, Gordon JI, Jansson JK, Knight R. Diversity, stability and resilience of the human gut microbiota. Nature 2012; 489:220–230 [View Article] [PubMed]
     [Google Scholar]
  2. Coretti L, Papato L, Riccio MP, Amato F, Cuomo M et al. Gut microbiota features in young children with autism spectrum disorders. Front Microbiol 2018; 9:3146 [View Article] [PubMed]
     [Google Scholar]
  3. Liu C, Finegold SM, Song Y, Lawson PA. Reclassification of Clostridium coccoides, Ruminococcus hansenii, Ruminococcus hydrogenotrophicus, Ruminococcus luti, Ruminococcus productus and Ruminococcus schinkii as Blautia coccoides gen. nov., comb. nov., Blautia hansenii comb. nov., Blautia hydrogenotrophica comb. nov., Blautia luti comb. nov., Blautia producta comb. nov., Blautia schinkii comb. nov. and description of Blautia wexlerae sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2008; 58:1896–1902 [View Article] [PubMed]
     [Google Scholar]
  4. Paek J, Shin Y, Kook JK, Chang YH. Blautia argi sp. nov., a new anaerobic bacterium isolated from dog faeces. Int J Syst Evol Microbiol 2019; 69:33–38 [View Article] [PubMed]
     [Google Scholar]
  5. Lagkouvardos I, Pukall R, Abt B, Foesel BU, Meier-Kolthoff JP et al. The Mouse Intestinal Bacterial Collection (miBC) provides host-specific insight into cultured diversity and functional potential of the gut microbiota. Nat Microbiol 2016; 1:16131 [View Article]
     [Google Scholar]
  6. Kaneuchi C, Benno Y, Mitsuoka T. Clostridium cocoides, a new species from the feces of mice. Int J Syst Bacteriol 1976; 26:482–486 [View Article]
     [Google Scholar]
  7. Kim JS, Park JE, Lee KC, Choi SH, Oh BS et al. Blautia faecicola sp. nov., isolated from faeces from a healthy human. Int J Syst Evol Microbiol 2020; 70:2059–2065 [View Article] [PubMed]
     [Google Scholar]
  8. Park SK, Kim MS, Bae JW. Blautia faecis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2013; 63:599–603 [View Article] [PubMed]
     [Google Scholar]
  9. Furuya H, Ide Y, Hamamoto M, Asanuma N, Hino T. Isolation of a novel bacterium, Blautia glucerasei sp. nov., hydrolyzing plant glucosylceramide to ceramide. Arch Microbiol 2010; 192:365–372 [View Article] [PubMed]
     [Google Scholar]
  10. Ezaki T, Li N, Hashimoto Y, Miura H, Yamamoto H. 16S ribosomal DNA sequences of anaerobic cocci and proposal of Ruminococcus hansenii comb. nov. and Ruminococcus productus comb. nov. Int J Syst Evol Microbiol 1994; 44:130–136
     [Google Scholar]
  11. Shin NR, Kang W, Tak EJ, Hyun DW, Kim PS et al. Blautia hominis sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2018; 68:1059–1064 [View Article] [PubMed]
     [Google Scholar]
  12. Bernalier A, Willems A, Leclerc M, Rochet V, Collins MD. Ruminococcus hydrogenotrophicus sp. nov., a new H2/CO2-utilizing acetogenic bacterium isolated from human feces. Arch Microbiol 1996; 166:176–183 [View Article] [PubMed]
     [Google Scholar]
  13. Simmering R, Taras D, Schwiertz A, Le Blay G, Gruhl B et al. Ruminococcus luti sp. nov., isolated from a human faecal sample. Syst Appl Microbiol 2002; 25:189–193 [View Article] [PubMed]
     [Google Scholar]
  14. Moore WEC, Johnson JL, Holdeman LV. Emendation of Bacteriodaceae and Butyrivibrio and descriptions of Desulfomonas gen. nov. and ten new species in the genera Desulfomonas, Butyrivibrio, Eubacterium, Clostridium, and Ruminococcus. Int J Syst Bacteriol 1976; 26:238–252 [View Article]
     [Google Scholar]
  15. Lawson PA, Finegold SM. Reclassification of Ruminococcus obeum as Blautia obeum comb. nov. Int J Syst Evol Microbiol 2015; 65:789–793 [View Article] [PubMed]
     [Google Scholar]
  16. Rieu-Lesme F, Morvan B, Collins MD, Fonty G, Willems A. A new H2/CO2-using acetogenic bacterium from the rumen: description of Ruminococcus schinkii sp. nov. FEMS Microbiol Lett 1996; 140:281–286 [View Article] [PubMed]
     [Google Scholar]
  17. Park SK, Kim MS, Roh SW, Bae JW. Blautia stercoris sp. nov., isolated from human faeces. Int J Syst Evol Microbiol 2012; 62:776–779 [View Article] [PubMed]
     [Google Scholar]
  18. Weisburg WG. 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 1991; 173:697–703 [View Article] [PubMed]
     [Google Scholar]
  19. Kumar S, Stecher G, Tamura K. MEGA7: Molecular Evolutionary Genetics Analysis version 7. 0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article] [PubMed]
     [Google Scholar]
  20. Larkin MA, Blackshields G, Brown NP, Chenna R, Mcgettigan PA et al. Clustal W and Clustal X version 2.0. Bioinformatics 2007; 23:2947–2948 [View Article] [PubMed]
     [Google Scholar]
  21. Saitou N, Nei M. The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4:406–425 [View Article] [PubMed]
     [Google Scholar]
  22. Felsenstein J. PHYLIP―Phylogeny Inference Package (Version 3.2. Cladistics 1989; 5:163–166
     [Google Scholar]
  23. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Systematic Biology 1971; 20:406–416 [View Article]
     [Google Scholar]
  24. Kimura M. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 1980; 16:111–120 [View Article] [PubMed]
     [Google Scholar]
  25. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39:783–791
     [Google Scholar]
  26. Yoon S-H, Ha S-M, Lim J, Kwon S, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie van Leeuwenhoek 2017; 110:1281–1286 [View Article] [PubMed]
     [Google Scholar]
  27. Meier-Kolthoff JP, Auch AF, Klenk HP, Goker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article] [PubMed]
     [Google Scholar]
  28. Delcher AL, Bratke KA, Powers EC, Salzberg SL. Identifying bacterial genes and endosymbiont DNA with Glimmer. Bioinformatics 2007; 23:673–679 [View Article] [PubMed]
     [Google Scholar]
  29. Hucker GJ. A new modification and application of the Gram stain. J Bacteriol 1921; 6:395–397 [View Article] [PubMed]
     [Google Scholar]
  30. Cerny G. Studies on the aminopeptidase test for the distinction of Gram-negative from Gram-positive bacteria. European J Appl Microbiol Biotechnol 1978; 5:113–122 [View Article]
     [Google Scholar]
  31. Wang K, Bao L, Ma K, Zhang J, Chen B et al. A novel class of α-glucosidase and HMG-CoA reductase inhibitors from Ganoderma leucocontextum and the anti-diabetic properties of ganomycin I in KK-Aymice. Eur J Med Chem 2017; 127:1035–1046 [View Article] [PubMed]
     [Google Scholar]
  32. Minnikin DE, O’Donnell AG, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
     [Google Scholar]
  33. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE: MIDI inc; 1990
     [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.005005
Loading
Blautia intestinalis sp. nov., isolated from human feces
Int J Syst Evol Microbiol 71, 005005 (2021); https://doi.org/10.1099/ijsem.0.005005
/content/journal/ijsem/10.1099/ijsem.0.005005
/content/journal/ijsem/10.1099/ijsem.0.005005
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error