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Volume 71, Issue 9

sp. nov., a novel actinomycete with antifungal activity against No Access

Abstract

The fungal pathogen is the causal agent of devastating gray mold diseases in many economically important fruits, vegetables, and flowers, leading to serious economic losses worldwide. In this study, a novel actinomycete NEAU-LD23 exhibiting antifungal activity against was isolated, and its taxonomic position was evaluated using a polyphasic approach. Based on the genotypic, phenotypic and chemotaxonomic data, it is concluded that the strain represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is NEAU-LD23 (=CCTCC AA 2019029=DSM 109824). In addition, strain NEAU-LD23 showed a strong antagonistic effect against (82.6±2.5%) and varying degrees of inhibition on nine other phytopathogenic fungi. Both cell-free filtrate and methanol extract of mycelia of strain NEAU-LD23 significantly inhibited mycelial growth of . To preliminarily explore the antifungal mechanisms, the genome of strain NEAU-LD23 was sequenced and analyzed. AntiSMASH analysis led to the identification of several gene clusters responsible for the biosynthesis of bioactive secondary metabolites with antifungal activity, including 9-methylstreptimidone, echosides, anisomycin, coelichelin and desferrioxamine B. Overall, this research provided us an excellent strain with considerable potential to use for biological control of tomato gray mold.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Botrytis cinerea , genomic properties , grey mould , polyphasic approach and Streptomyces botrytidirepellens sp. nov.
Funding
This study was supported by the:
  • the National Natural Science Foundation of China (Award 31972291)
    • Principle Award Recipient: WangXiangjing
© 2021 The Authors
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2021-09-14
2024-04-25
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Streptomyces botrytidirepellens sp. nov., a novel actinomycete with antifungal activity against Botrytis cinerea
Int J Syst Evol Microbiol 71, 005004 (2021); https://doi.org/10.1099/ijsem.0.005004
/content/journal/ijsem/10.1099/ijsem.0.005004
/content/journal/ijsem/10.1099/ijsem.0.005004
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