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Abstract

Strain F2A, isolated from the cricket , was subjected to a polyphasic taxonomic characterization. Cells of the strain were rod-shaped, Gram-stain-negative and catalase- and oxidase-positive. It did not assimilate any carbohydrates. The strain's 16S rRNA gene sequence showed highest similarity to QZS01 (96.4 %). The next highest similarity values were found to representatives of related genera (<93 %). The genome size of strain F2A was 3.2 Mbp and the G+C content was 36.4 mol%. Average nucleotide identity values based on and MUMmer and average amino acid identity values between strain F2A and QZS01 were 74.29/74.43, 83.88 and 74.70 %, respectively. The quinone system predominantly contained ubiquinone Q-8. In the polar lipid profile, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified phospholipid were detected. The polyamine pattern consisted of the major compounds putrescine and spermidine. Major fatty acids were C ω7 and C and the hydroxyl acids were C 3-OH, C 2-OH and C 3-OH. The diagnostic diamino acid of the peptidoglycan was -diaminopimelic acid. Due to its association with the only species of the genus but its distinctness from we here propose the novel species sp. nov. F2A (=CCM 9136=LMG 32211).

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2021-09-13
2021-09-24
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