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Abstract

A Gram-stain-negative, coccus-shaped, obligately anaerobic, non-motile and non-spore-forming bacterium, designated strain JN500902, was isolated from the mud in a fermentation cellar used continuously over 30 years for Chinese strong-flavour production. Colonies were white, circular, convex and smooth-edged. Growth was observed at 20–40 °C (optimum, 37 °C), at pH 5.0–10 (optimum, pH 7.5), with 0–2 % (w/v) NaCl and with 0–4 % (v/v) ethanol. The Biolog assay demonstrated positive reactions of strain JN500902 in the metabolism of -fucose and pyruvate. The predominant cellular fatty acids (>10 %) consisted of C and C. The major end metabolites of strain JN500902 were acetic acid and ethanol when incubated anaerobically in liquid reinforced clostridial medium. Acetate was the major organic acid end product. The complete genome size of strain JN500902 was 3 420 321 bp with 3327 identified genes. The G+C content was 43.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences affiliated strain JN500902 with the family , having low sequence similarity (92.8 %) to the nearest type strain, DSM 3224 and forming a clearly distinct branch. Core genome phylogenetic analysis of the isolate and 134 strains belonging to the family also revealed that strain JN500902 was well-separated from other genera of this family as a monophyletic clade. The average nucleotide identity and amino acid identity values between strain JN500902 and 134 strains were less than 74 and 65 %, respectively. Considering its polyphasic characteristics, strain JN500902 represents a novel genus and species within the family , for which the name gen. nov., sp. nov. is proposed. The type strain is JN500902 (=CICC 24502=JCM 33939).

Funding
This study was supported by the:
  • National First-Class Discipline Programme of Light Industry Technology and Engineering (Award LITE2018-11)
    • Principle Award Recipient: Zheng-HongXu
  • Fundamental Research Funds for the Central Universities (Award JUSRP12055)
    • Principle Award Recipient: Li-JuanChai
  • Postdoctoral Research Foundation of China (Award 2020M671407)
    • Principle Award Recipient: Li-JuanChai
  • National Natural Science Foundation of China (Award 31901658)
    • Principle Award Recipient: Li-JuanChai
  • National Key Research and Development Programme of China (Award 2018YFC1603800)
    • Principle Award Recipient: Zhen-MingLu
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2021-09-16
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