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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 9

gen. nov., sp. nov., a new member of suborder isolated from High Arctic tundra soil Free

Abstract

A yellow bacterial strain, designated LRZ-2, was isolated from High Arctic tundra near the settlement Ny-Ålesund in the Svalbard Archipelago, Norway. The cells were Gram-stain-positive, aerobic and non-sporulating. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain LRZ-2 represented a novel member of the suborder . Its nearest phylogenetic neighbours were the members of the genus , with 16S rRNA gene sequence similarity of 95.3–96.9 %. The average nucleotide identity and digital DNA–DNA hybridization values between the genomes of strain LRZ-2 and its closely related strains were 77.4–74.3 % and 21.4–19.6 %, respectively. The DNA G+C content was 72.4 mol%. The peptidoglycan type of the isolate was A4β with an interpeptide bridge comprising -ornithine and -glutamic acid. The predominant menaquinone was MK-9 (H) and the major fatty acids were anteiso-C, C, anteiso-C A, anteiso-C and iso-C. The polar lipids were diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, phosphatidylinositol dimannoside, unidentified phosphoglycolipid, four unidentified phospholipids and two unidentified polar lipids. Strain LRZ-2 showed a 16S rRNA gene signature pattern consisting of nucleotides at positions 120 (A), 131–231 (C-G), 196 (C), 342–347 (C-G), 444–490 (A-U), 580–761 (C-G), 602–636 (C-G), 670–736 (A-U), 822–878 (G-C), 823–877 (G-C), 826–874 (C-G), 827 (U), 843 (C), 950–1231 (U-A), 1047–1210 (G-C), 1109 (C), 1145 (G), 1309–1328 (G-C), 1361 (G) and 1383 (C), which clearly distinguished it from all genera previously reported in the suborder . On the basis of the phylogenetic, phenotypic and chemotaxonomic data, strain LRZ-2 is considered to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is LRZ-2 (=CCTCC AB 2012163=DSM 100332).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): high-Arctic tundra soil , Micrococcineae , Pengzhenrongella sicca , polyphasic approach and Svalbard Archipelago
Funding
This study was supported by the:
  • Key Project of Chinese Ministry of Education (Award V201308)
    • Principle Award Recipient: FangPeng
  • National Natural Science Foundation of China (Award 31200038)
    • Principle Award Recipient: FangPeng
  • R & D Infrastructure and Facility Development Program from the Ministry of Science and Technology of the People’s Republic of China (Award NIMR-2014-8)
    • Principle Award Recipient: FangPeng
  • National Basic Research Program of China (973 Program) (Award 2011CB808800)
    • Principle Award Recipient: FangPeng
© 2021 The Authors
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2021-09-24
2025-03-20
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Pengzhenrongella sicca gen. nov., sp. nov., a new member of suborder Micrococcineae isolated from High Arctic tundra soil
Int J Syst Evol Microbiol 71, 004988 (2021); https://doi.org/10.1099/ijsem.0.004988
/content/journal/ijsem/10.1099/ijsem.0.004988
/content/journal/ijsem/10.1099/ijsem.0.004988
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