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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 8

sp. nov., isolated from the deep-sea cold seep sediment of South China Sea Free

Abstract

In this study, we report a Gram-stain-negative, rod-shaped, non-pigmented, motile and aerobic bacterium named strain MTZ26, which was isolated from deep-sea sediment sampled at a cold seep in the South China Sea. Growth of strain MTZ26 occurred at 4–40 °C (optimum, 25–30 °C), pH 6.0–10.0 (optimum, 7.0–8.0) and with 1.0–11.0 % (w/v) NaCl (optimum, 6.0–8.0 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MTZ26 belonged to the genus and was closely related to PSC101 (97.5 % sequence similarity) and KDW4 (97.5 %). Genomic analysis indicated that strain MTZ26 contains a circular chromosome of 3 331 814 bp with G+C content of 42.2 mol%. The predominant respiratory quinone of MTZ26 was ubiquinone-8. The polar lipids of MTZ26 contained phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminophospholipid and one unidentified phospholipid. The major fatty acids of strain MTZ26 contained C C C, C ω8, C 3-OH, C 3-OH, C ω8 and summed feature 8 (C ω7 or/and C ω6). Results of phylogenetic, physiological, biochemical and morphological analyses suggested that strain MTZ26 represents a novel species of the genus , and the name sp. nov. is proposed with the type strain MTZ26 (=MCCC 1K05568=JCM 34321).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Alphaproteobacteria , cold seep , Psychrosphaera and sediment
Funding
This study was supported by the:
  • National Key R&D Program of China (Award 2018YFC0310603)
    • Principle Award Recipient: ZhangJian
  • National Key R&D Program of China (Award 2018YFC0310801)
    • Principle Award Recipient: SunQing-lei
© 2021 Yantai University Yantai, Shandong CHINA
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/content/journal/ijsem/10.1099/ijsem.0.004983
2021-08-25
2025-01-26
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Psychrosphaera ytuae sp. nov., isolated from the deep-sea cold seep sediment of South China Sea
Int J Syst Evol Microbiol 71, 004983 (2021); https://doi.org/10.1099/ijsem.0.004983
/content/journal/ijsem/10.1099/ijsem.0.004983
/content/journal/ijsem/10.1099/ijsem.0.004983
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