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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 8

Proposal of subsp. Varsha and Nampoothiri 2016 as a later heterotypic synonym of Chen . 2014 and subsp. comb. nov. Free

Abstract

Currently, contains two subspecies: subsp. and subsp. . In a study by Varsha and Nampoothiri, high (99.7 %) and (99.6 %) sequence similarities indicated that subsp. and probably have a close taxonomic relationship; low (92.2 %) and (97.8 %) sequence similarities and relatively low DNA–DNA hybridization value (75.8 %) indicated that subsp. and subsp. probably represent two different species. In the present study, the taxonomic relationships between subsp. , subsp. and were re-examined based on sequence analyses of 16S rRNA, , , and genes, average nucleotide identity (ANI), digital DNA–DNA hybridization (dDDH) values, average amino acid identity (AAI), fatty acid methyl ester analysis and phenotypic characterization. subsp. LMG 30663 exhibited 97.3 % ANI, 78.3 % dDDH and 96.4 % AAI values to NBRC 109475, higher than the threshold for species demarcation (95–96, 70 and 95–96 %, respectively), indicating that subsp. LMG 30663 and NBRC 109475 belong to the same species. subsp. LMG 30663 had 91.2 % ANI, 43.3 % dDDH and 92.9–93.0% AAI values with the type strain of subsp. , indicating that they represent two different species. Because has been proposed and validated before subsp. , subsp. is transferred to as subsp. comb. nov. The type strain of subsp. is BSN307 (=DSM 100577=MCC 2824=KCTC 21083=LMG 30663). The type strain of subsp. is 516 (=NBRC 109475=BCRC 80576).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): genome , Lactococcus formosensis , Lactococcus garvieae subsp. bovis and reclassification
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award no. 31471594)
    • Principle Award Recipient: Tao GuChun
© 2021 The Authors
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2021-08-24
2025-01-19
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Proposal of Lactococcus garvieae subsp. bovis Varsha and Nampoothiri 2016 as a later heterotypic synonym of Lactococcus formosensis Chen et al. 2014 and Lactococcus formosensis subsp. bovis comb. nov.
Int J Syst Evol Microbiol 71, 004980 (2021); https://doi.org/10.1099/ijsem.0.004980
/content/journal/ijsem/10.1099/ijsem.0.004980
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