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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 8

sp. nov., an actinomycete isolated from human skin No Access

Abstract

A novel actinomycete, designated as strain WCH-YHL-001, was isolated from skin biopsy specimens of a patient at West China Hospital, Chengdu, Sichuan Province, PR China. The cells were Gram-positive, aerobic, heterotrophic and non-motile. They formed an extensive substrate with short aerial mycelia, whose branches fragmented into rod-shaped elements. Growth occurred at 10–40 °C, pH 5.0–12.0 and with NaCl concentrations of 0–4.0 % (w/v). The major cellular fatty acids of strain WCH-YHL-001 were C, C 9, C 10-methyl and summed feature 3. The predominant respiratory quinone was MK-8 (H4-cycl). The major polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol mannoside, unknown phospholipids and unidentified glycolipids. The diagnostic diamino acid of peptidoglycan was -diaminopimelic acid. The whole-cell sugar pattern consisted of arabinose and glucose. Phylogenetic analysis based on the 16S rRNA gene sequence indicated that strain WCH-YHL-001 belonged to the genus . The average nucleotide identity (ANI) and DNA–DNA hybridization (DDH) values between strain WCH-YHL-001 and type strains of species were lower than the cut-offs (≥95–96 % for ANI and ≥70 % for DDH) required to define a bacterial species. The genomic DNA G+C content was 67.8 mol%. Phylogenetic, physiological and chemotaxonomic data suggested that strain WCH-YHL-001 represented a novel species of the genus , for which the name sp. nov. is proposed, with the type strain WCH-YHL-001 (=GDMCC 4.181=JCM 34475 =NBRC 114973).

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Keyword(s): Nocardia huaxiensis sp. nov. , polyphasic taxonomy and skin sample
© 2021 The Authors
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2021-08-23
2024-04-20
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Nocardia huaxiensis sp. nov., an actinomycete isolated from human skin
Int J Syst Evol Microbiol 71, 004970 (2021); https://doi.org/10.1099/ijsem.0.004970
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