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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 8

gen. nov., sp. nov., a new member of the family , isolated from marine sediment Free

Abstract

A Gram-stain-negative, rod-shaped and facultatively aerobic bacterial strain, designated F7430, was isolated from coastal sediment collected at Jingzi Wharf in Weihai, PR China. Cells of strain F7430 were 0.3–0.4 µm wide, 2.0–2.6 µm long, non-flagellated, non-motile and formed pale-beige colonies. Growth was observed at 4–40 °C (optimum, 30 °C), pH 6.0–9.0 (optimum, pH 7.5–8.0) and at NaCl concentrations of 1.0–10.0 % (w/v; optimum, 1.0 %). The sole respiratory quinone of strain F7430 was ubiquinone 8 and the predominant cellular fatty acids were summed feature 8 (C 7 / C 6; 60.7 %), summed feature 3 (C 7/C 6; 30.2 %) and C iso (13.9 %). The polar lipids of strain F7430 consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, one unidentified phospholipid and three unidentified lipids. Results of 16S rRNA gene sequences analyses indicated that this strain belonged to the family and had high sequence similarities to JCM 51547 (95.3 %) and DSM 27932 (95.2 %) followed by 92.9–95.0 % sequence similarities to other type species within the aforementioned family. The gene sequences analyses indicated that the novel strain had the highest sequence similarities to JCM 51547 (82.2 %) and DSM 21924 (82.2 %) followed by 75.2–80.5 % sequence similarities to other type species within this family. Phylogenetic analyses showed that strain F7430 constituted a monophyletic branch clearly separated from the other genera of family . Whole-genome sequencing of strain F7430 revealed a 3.3 Mbp genome size with a DNA G+C content of 52.6 mol%. The genome encoded diverse metabolic pathways including the Entner–Doudoroff pathway, assimilatory sulphate reduction and biosynthesis of dTDP--rhamnose. Based on results from the current polyphasic study, strain F7430 is proposed to represent a novel species of a new genus within the family , for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is F7430 (=KCTC 72873=MCCC 1H00420).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): 16S rRNA gene sequence , genome information , marine sediment , phylogenetic analysis , RpoB protein sequence , Sediminihaliea albiluteola and taxonomy
Funding
This study was supported by the:
  • Natural Science Foundation of Jilin Province (Award 32070002, 31770002)
    • Principle Award Recipient: DuZong-Jun
© 2021 The Authors
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2021-08-16
2025-01-20
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Sediminihaliea albiluteola gen. nov., sp. nov., a new member of the family Halieaceae, isolated from marine sediment
Int J Syst Evol Microbiol 71, 004959 (2021); https://doi.org/10.1099/ijsem.0.004959
/content/journal/ijsem/10.1099/ijsem.0.004959
/content/journal/ijsem/10.1099/ijsem.0.004959
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