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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 8

sp. nov., a slow-growing bacterium isolated from rhizosphere of rice plant and emended description of the genus No Access

Abstract

In the present study, in an attempt to explore the diversity of bacteria in the roots of rice plants, a Gram-stain-negative, motile, facultatively anaerobic, non-pigmented, catalase-positive, oxidase-negative and rod-shaped bacterium with polar flagella was isolated. Phylogenetic analysis based on 16S rRNA gene sequences revealed highest sequence similarity to KCTC 42859 (98.2%) followed by KCTC 42562 (98%), II-D5 (97.9%) and MWH-BRAZ-DAM2D (97.4%). Growth of strain JUR4 occurred at 10–37 °C (optimum, 30 °C), at pH 5.5–8.0 (optimum, 6.5–7) and in the presence of 0–0.2% NaCl (optimum, 0%, w/v). The genome size of strain JUR4 was found to be 3.34 Mb containing 3139 predicted protein-coding genes with a DNA G+C content of 61.5 mol%. The digital DNA–DNA hybridization and average nucleotide identity values between the genome sequence of strain JUR4 and closely related reference strains were 21.0–24.8% and 74.7–81.4%, respectively. Strain JUR4 contained diphosphatidylglycerol, phoshatidylethanolamine, one unidentified phosphoglycolipid, one unidentified aminophosphoglycolipid, one unidentified phospholipid and seven unidentified glycolipids. The major fatty acids were C and summed feature 3 (comprising C 7 and/or C 6), and ubiquinone Q-8 was the sole isoprenoid quinone. So far, all species belonging to the genus have been described as non-motile and devoid of flagella. All species were isolated from freshwater and are therefore denoted as planktonic bacteria. This present study introduces a novel motile member of isolated from the root of rice plant, and introduces the genes associated with motility and methyl-accepting chemotaxis proteins. Phylogenetic, phenotypic, chemotaxonomic and genotypic data clearly indicates that strain JUR4 represents a novel species of the genus for which the name sp. nov. is proposed. The type strain is JUR4 (=KACC 21745=NBRC 114484).

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Keyword(s): Limnohabitans radicicola , non-pigmented , polar flagella and rhizosphere
© 2021 The Authors
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2021-08-17
2024-04-25
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Limnohabitans radicicola sp. nov., a slow-growing bacterium isolated from rhizosphere of rice plant and emended description of the genus Limnohabitans
Int J Syst Evol Microbiol 71, 004957 (2021); https://doi.org/10.1099/ijsem.0.004957
/content/journal/ijsem/10.1099/ijsem.0.004957
/content/journal/ijsem/10.1099/ijsem.0.004957
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