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Volume 71, Issue 8

sp. nov., a novel genistein-producing actinomycete isolated from a Chinese medicinal plant and proposal of Corbaz . 1957 as a later heterotypic synonym of Arishima . 1956 No Access

Abstract

A novel genistein-producing actinobacterial strain, designated strain CRPJ-33, was isolated from the healthy leaves of a medicinal plant collected from Hunan Province, PR China. 16S rRNA gene sequence analysis indicated strain CRPJ-33 belonged to the genus and had 99.7, 99.0, 98.9, 98.9, 98.8 and 98.7% sequence similarities to YIM T102, NRRL B-1676, NRRL B-5410, NBRC 12797, LMG 20100 and NBRC 12882, respectively. Phylogenetic analysis of 16S rRNA gene sequences showed that strain CRPJ-33 was most closely related to YIM T102. However, digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between them were much less than the recommended threshold values. Furthermore, differential comparisons of the phenotypic characteristics were enough to distinguish strain CRPJ-33 from YIM T102. Meanwhile, the ANI and dDDH values or MLSA distances between strain CRPJ-33 and other type strains, which exhibited ≥98.7 % 16S rRNA gene sequence similarities to strain CRPJ-33, were far away from the recommended threshold values. Based on these results, it is thought that strain CRPJ-33 should represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CRPJ-33 (=MCCC 1K04965=JCM 34526). In addition, the phenotypic, chemotaxonomic and genotypic characteristics, as well as phylogenetic information revealed that the type strains of and should belong to same genomic species. Consequently, it is proposed that is a heterotypic synonym of for which an emended description is given.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): heterotypic synonymm , medicinal plant , polyphasic taxonomy and Streptomyces genisteinicus sp. nov.
Funding
This study was supported by the:
  • Hunan Provincial Science and Technology Department (Award 20A200)
    • Principle Award Recipient: JianGao
  • National Key R and D Program of China (Award 2017YFE0117600)
    • Principle Award Recipient: TangXinke
© 2021 The Authors
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2021-08-12
2024-04-24
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Streptomyces genisteinicus sp. nov., a novel genistein-producing actinomycete isolated from a Chinese medicinal plant and proposal of Streptomyces michiganensis Corbaz et al. 1957 as a later heterotypic synonym of Streptomyces xanthochromogenes Arishima et al. 1956
Int J Syst Evol Microbiol 71, 004954 (2021); https://doi.org/10.1099/ijsem.0.004954
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