1887

Abstract

Two pairs of aerobic, Gram-stain-positive, rod-shaped strains (HY164/HY044, HY168/HY211) were isolated from bat faecal samples. Strains HY164 and HY044 were motile with a polar flagellum, and had 16S rRNA gene similarity of 95.1–98.6 % to YIM 93306 and T3246-1; strains HY168 and HY211 were most similar to DSM 18029 (96.6 %). Phylogenetic trees based on 16S rRNA gene and whole genome sequences revealed affiliation of strains HY164 and HY168 to the family , representing novel lineages in the genera and , respectively, which was also supported by the results for average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH). For all isolates, the principal cellular fatty acids were -C and -C. HY164 and HY168 had MK-8(H) as the predominant isoprenoid quinone, diphosphatidylglycerol, phosphatidylglycerol, several unidentified phospholipids and glycolipids as common polar lipids while the latter strain additionally contained one unidentified aminophospholipid and one unidentified phosphoglycolipid. Besides sharing alanine, glutamic acid and lysine with HY164, HY168 additionally contained 2,4-diaminobutyric acid in the cell-wall peptidoglycan. The whole-cell sugars of HY164 were ribose and rhamnose, while HY168 only included the latter. The DNA G+C contents of HY164 and HY168 were 71.0 and 69.1 mol%, respectively. Combining the polyphasic taxonomic data, HY164 (=CGMCC 4.7606=JCM 33464) is classified as representing a novel species of the genus with the proposed name sp. nov., and HY168 (=CGMCC 1.16970=JCM 33465) is proposed to represent a novel species of the genus with the name sp. nov.

Funding
This study was supported by the:
  • research units of discovery of unknown bacteria and function (Award 2018RU010)
    • Principle Award Recipient: JianguoXu
  • national key r&d program of china (Award 2019YFC1200505)
    • Principle Award Recipient: JingYang
  • national key r&d program of china (Award 2019YFC1200501)
    • Principle Award Recipient: JingYang
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/content/journal/ijsem/10.1099/ijsem.0.004953
2021-08-13
2024-03-28
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