1887

Abstract

Three highly alkaliphilic bacterial strains designated as A1, H1 and B1 were isolated from two highly alkaline springs at The Cedars, a terrestrial serpentinizing site. Cells from all strains were motile, Gram-negative and rod-shaped. Strains A1, H1 and B1 were mesophilic (optimum, 30 °C), highly alkaliphilic (optimum, pH 11) and facultatively autotrophic. Major cellular fatty acids were saturated and monounsaturated hexadecenoic and octadecanoic acids. The genome size of strains A1, H1 and B1 was 2 574 013, 2 475 906 and 2 623 236 bp, and the G+C content was 66.0, 66.2 and 66.1 mol%, respectively. Analysis of the 16S rRNA genes showed the highest similarity to the genera (95.1–96.4 %), (93.0–93.6 %) and (93.0–96.6 %) in the family . Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis based on core gene sequences revealed that the isolated strains diverged from the related species, forming a distinct branch. Average amino acid identity values of strains A1, H1 and B1 against the genomes of related members in this family were below 67 %, which is below the suggested threshold for genera boundaries. Average nucleotide identity by values and digital DNA–DNA hybridization among the three strains were below 92.0 and 46.6 % respectively, which are below the suggested thresholds for species boundaries. Based on phylogenetic, genomic and phenotypic characterization, we propose gen. nov., sp. nov. (type strain A1=NBRC 111848=DSM 103917), sp. nov. (type strain H1= NBRC 111849=DSM 103920) and sp. nov. (type strain B1=NBRC 111850=DSM 103919) belonging to the family . We have designated the type species for the genus because it is the dominant species in The Cedars springs.

Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 18H02501)
    • Principle Award Recipient: ShinoSuzuki
  • National Science Foundation (Award 1424711)
    • Principle Award Recipient: NealsonH. Kenneth
  • National Science Foundation (Award 1424646)
    • Principle Award Recipient: ShinoSuzuki
  • Japan Society for the Promotion of Science (Award 20H04620)
    • Principle Award Recipient: ShinoSuzuki
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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2021-08-11
2024-03-29
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