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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 8

gen. nov., sp. nov., sp. nov. and sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs Open Access

Abstract

Three highly alkaliphilic bacterial strains designated as A1, H1 and B1 were isolated from two highly alkaline springs at The Cedars, a terrestrial serpentinizing site. Cells from all strains were motile, Gram-negative and rod-shaped. Strains A1, H1 and B1 were mesophilic (optimum, 30 °C), highly alkaliphilic (optimum, pH 11) and facultatively autotrophic. Major cellular fatty acids were saturated and monounsaturated hexadecenoic and octadecanoic acids. The genome size of strains A1, H1 and B1 was 2 574 013, 2 475 906 and 2 623 236 bp, and the G+C content was 66.0, 66.2 and 66.1 mol%, respectively. Analysis of the 16S rRNA genes showed the highest similarity to the genera (95.1–96.4 %), (93.0–93.6 %) and (93.0–96.6 %) in the family . Phylogenetic analysis based on 16S rRNA gene and phylogenomic analysis based on core gene sequences revealed that the isolated strains diverged from the related species, forming a distinct branch. Average amino acid identity values of strains A1, H1 and B1 against the genomes of related members in this family were below 67 %, which is below the suggested threshold for genera boundaries. Average nucleotide identity by values and digital DNA–DNA hybridization among the three strains were below 92.0 and 46.6 % respectively, which are below the suggested thresholds for species boundaries. Based on phylogenetic, genomic and phenotypic characterization, we propose gen. nov., sp. nov. (type strain A1=NBRC 111848=DSM 103917), sp. nov. (type strain H1= NBRC 111849=DSM 103920) and sp. nov. (type strain B1=NBRC 111850=DSM 103919) belonging to the family . We have designated the type species for the genus because it is the dominant species in The Cedars springs.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): alkaliphile , autotrophic growth , Serpentinization and Serpentinomonas/Serpentinimonas
Funding
This study was supported by the:
  • Japan Society for the Promotion of Science (Award 18H02501)
    • Principle Award Recipient: ShinoSuzuki
  • National Science Foundation (Award 1424711)
    • Principle Award Recipient: NealsonH. Kenneth
  • National Science Foundation (Award 1424646)
    • Principle Award Recipient: ShinoSuzuki
  • Japan Society for the Promotion of Science (Award 20H04620)
    • Principle Award Recipient: ShinoSuzuki
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License.
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Serpentinimonas gen. nov., Serpentinimonas raichei sp. nov., Serpentinimonas barnesii sp. nov. and Serpentinimonas maccroryi sp. nov., hyperalkaliphilic and facultative autotrophic bacteria isolated from terrestrial serpentinizing springs
Int J Syst Evol Microbiol 71, 004945 (2021); https://doi.org/10.1099/ijsem.0.004945
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