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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 8

sp. nov., a facultative anaerobic bacterium isolated from mangrove sediment Free

Abstract

Two Gram-stain-negative, motile, facultatively anaerobic, rod-shaped strains, FJAT-53720 and FJAT-53726, were isolated from rhizosphere sediment of plant . Phylogenetic analysis based on their 16S rRNA gene sequences revealed that strains FJAT-53720 and FJAT-53726 were affiliated to the genus (forming an independent cluster) with the highest sequence similarity to the type strain of . Optimum growth of both strains was observed at 30 °C and pH 7. The respiratory quinones were Q-7, Q-8 and MK-7. The polar lipid profile included phosphatidylmethyl ethanolamine, phosphatidylethanolamine, diphosphatidylglycerol, one unidentified aminophospholipid and four unknown phospholipids. The major fatty acids of strains FJAT-53720 and FJAT-53726 were iso-C C 8 and summed feature 3. The genomic DNA G+C content of strain FJAT-53720 was 55.6 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between FJAT-53720 and FJAT-53726 were 97.4 and 77.9 %, confirming that they were similar species and hence FJAT-53720 was selected for further analysis. The ANI and dDDH values between FJAT-53720 and other members of the genus were below the cut-off level (95–96 %; 70 %) for species delineation. Based on the above results, FJAT-53720 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is FJAT-53720 (=KCTC 82444=GDMCC 1.2207).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Cyperus malaccensis , facultative anaerobic , rhizosphere and Shewanella cyperi sp. nov
Funding
This study was supported by the:
  • Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory (Zhuhai) (Award 3110200005)
    • Principle Award Recipient: Wen-JunLi
  • Fujian Academy of Agricultural Sciences (Award GJPY2019003)
    • Principle Award Recipient: GuohongLiu
  • Fujian Special Fund for Scientific Research Institutes in the Public Interest (Award 2020R1034001)
    • Principle Award Recipient: GuohongLiu
© 2021 Sun Yat-Sen University, P.R. China
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2021-08-04
2024-12-08
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Shewanella cyperi sp. nov., a facultative anaerobic bacterium isolated from mangrove sediment
Int J Syst Evol Microbiol 71, 004940 (2021); https://doi.org/10.1099/ijsem.0.004940
/content/journal/ijsem/10.1099/ijsem.0.004940
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