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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 7

sp. nov., an epiphytic bacterium isolated from rice root surfaces No Access

Abstract

Two Gram-stain-negative, non-motile, rod-shaped bacterial strains were isolated from the surfaces of rice roots. They were designated as strains 1303 and 1310. Their colonies were circular, entire, opaque, convex and yellow. They were chitinase- and catalase-positive, reduced nitrate and grew at 16–37 °C (optimum, 30 °C), pH 5.0–10.0 (optimum, pH 7.0) and 0–2.0% NaCl (optimum, 1.0 %). Based on the 16S rRNA gene sequence analysis, they were classified as members of the genus . Results of phylogenetic and phylogenomic analyses indicated that they formed a cluster with YC6729, JN246, 10-7 W-9003 and G-6-1-13. When the genomic sequences of strains 1303 and 1310 were compared with their close relatives, the average nucleotide identity and digital DNA–DNA hybridization values were below the cut-off levels. Phosphatidylethanolamine was the major polar lipid. MK-7 was the major respiratory quinone. iso-C, C 5c, iso-C 3-OH and summed feature 3 (C 7c/C c) were the predominant fatty acids. Differential characteristics between both strains and their close relatives were also observed. Based on the distinctions in genotypic, phenotypic and chemotypic features, strains 1303 and 1310 represent members of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 1303 (=KACC 22075=TBRC 12926).

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  • Accepted:
  • Published Online:
Keyword(s): Chitinophaga , epiphyte and Oryza sativa
Funding
This study was supported by the:
  • king mongkut's institute of technology ladkrabang (Award 2563-02-05-31)
    • Principle Award Recipient: ChokchaiKittiwongwattana
© 2021 The Authors
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2021-07-28
2024-04-16
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Chitinophaga oryzae sp. nov., an epiphytic bacterium isolated from rice root surfaces
Int J Syst Evol Microbiol 71, 004926 (2021); https://doi.org/10.1099/ijsem.0.004926
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