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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 71, Issue 7

sp. nov., isolated from chromium-containing chemical plant soil Free

Abstract

A Gram-stain-positive, rod-shaped, aerobic, non-motile, non-sporulating bacterial strain, designated CSA1, was isolated from chromium-containing soil sampled at a chemical plant. Growth of strain CSA1 occurred at pH 6–10 (optimum, pH 7), 15–45 °C (optimum, 30 °C) and in the presence of 0.5–6.5 % (w/v) NaCl (optimum, 2 %). The 16S rRNA gene sequence of strain CSA1 revealed the highest similarity to A2 (97.5 %), K 70/01 (97.3 %), Re6 (96.6 %), F3-P9 (96.2 %), CC-MF41 (96.1 %) and S27 (96.0 %). The draft genome of CSA1 was approximately 3 350 931 bp in size with a G+C content of 70.6 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values among strain CSA1 and the selected species were 74.0–79.2 % (ANIb), 84.3–87.1 % (ANIm) and 21.5–25.4 % (dDDH), which are below the recommended cutoff values for species delineation. The major fatty acids were anteiso-C, iso-C and anteiso-C. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol and an unknown glycolipid. The predominant menaquinones were MK-11, MK-8 and MK-6. The cell-wall amino acids were 2,4-diaminobutyric acid, alanine, glycine, glutamic acid and threonine. From the phenotypic, chemotaxonomic and molecular features, strain CSA1 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CSA1 (=JCM 34359=CGMCC 1.18746).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): chromium soil , Leucobacter chromiisoli and novel species
Funding
This study was supported by the:
  • Key Research and Development Program of Jiangxi Province (Award 2020YFS0287)
    • Principle Award Recipient: YongqiangTian
  • National Key Research and Development Program of China (Award 2018YFC1802201)
    • Principle Award Recipient: YongqiangTian
© 2021 The Authors
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2021-07-28
2025-04-21
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Leucobacter chromiisoli sp. nov., isolated from chromium-containing chemical plant soil
Int J Syst Evol Microbiol 71, 004923 (2021); https://doi.org/10.1099/ijsem.0.004923
/content/journal/ijsem/10.1099/ijsem.0.004923
/content/journal/ijsem/10.1099/ijsem.0.004923
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