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Abstract

Two Gram-stain-negative, catalase-positive, oxidase-negative, rod-shaped, non-flagellated, non-spore-forming and non-motile strains (YJ13C and H41) were isolated from a mariculture fishpond in PR China. Comparisons based on 16S rRNA gene sequences indicated that YJ13C and H41 shared 16S rRNA gene sequences similarities between 92.6 and 99.2 % with species of the genus . YJ13C only shared 93.8 % 16S rRNA gene sequence similarity with H41. The reconstructed phylogenetic and phylogenomic trees indicated that YJ13C and H41 clustered closely with species of the genus . The calculated pairwise orthologous average nucleotide identity with (OrthoANIu) values between strains YJ13C and H41 and other related strains were all less than 79.5 %. The OrthoANIu value between YJ13C and H41 was only 69.9 %. MK-7 was the predominant respiratory quinone of YJ13C and H41 and their major cellular fatty acids contained iso-C, C ω7 and C ω9. The polar lipids profiles of YJ13C and H41 consisted of phosphatidylethanolamine and several kinds of unidentified lipids. Combining the above descriptions, strains YJ13C and H41 represent two distinct novel species of the genus , for which the names sp. nov. (type strain YJ13C=GDMCC 1.2178=KCTC 82450) and sp. nov. (type strain H41=GDMCC 1.2179=KCTC 82451) are proposed.

Funding
This study was supported by the:
  • gdas’ special project of science and technology development (Award 2020GDASYL-20200103016; 2020GDASYL-20200402001)
    • Principle Award Recipient: MeiyingXu
  • guangdong mepp fund (Award NO. GDOE(2019)A34)
    • Principle Award Recipient: MeiyingXu
  • guangdong provincial programs for science and technology development (Award 2020B0202080005; 2019B110205004)
    • Principle Award Recipient: MeiyingXu
  • guangdong technological innovation strategy of special funds (Award Key Areas of Research and Development Program (2018B020205003))
    • Principle Award Recipient: MeiyingXu
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2021-07-22
2021-07-29
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