A novel Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile, rod-shaped bacterium, designated strain YIM M12148T, was isolated from a marine sediment sample collected from the Indian Ocean. The strain grew optimally at 28 °C, pH 8.0 and in the presence of 1–3 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M12148T belongs to the genus Gulosibacter, with the highest sequence similarity to Gulosibacter faecalis NBRC 15706T (96.12 %). The cell-wall sugars of strain YIM M12148T were rhamnose, ribose, glucose and mannose. The predominant isoprenoid quinones were MK-8 and MK-9. The polar lipids consisted of major amounts of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unknown phospholipid and one unknown lipid. Major fatty acids (>5 % of the total) of the novel isolate were anteiso-C15 : 0, iso-C15 : 0, iso-C13 : 0 and anteiso-C13 : 0. The genomic DNA G+C content of strain YIM M12148T was 67.15 mol%. On the basis of genotypic and phenotypic data, it is apparent that strain YIM M12148T represents a novel species of the genus Gulosibacter, for which the name Gulosibacter sediminis sp. nov. is proposed. The type strain is YIM M12148T (=KCTC 29660T=DSM 29154T).
ParkMH, TraiwanJ, JungMY, KimW. Gulosibacter chungangensis sp. nov. an actinomycete isolated from a marine sediment, and emended description of the genus Gulosibacter. Int J Syst Evol Microbiol2012; 62:1055–1060 [View Article] [PubMed]
NouiouiI, CarroL, García-LópezM, Meier-KolthoffJP, WoykeT et al. Genome-based taxonomic classification of the phylum Actinobacteria. Front Microbiol2018; 9:2007 [View Article] [PubMed]
JiangY, TangSK, WieseJ, XuLH, ImhoffJ et al.Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces. Int J Syst Evol Microbiol2007; 57:2694–2698 [View Article]
LiWJ, XuP, SchumannP, ZhangYQ, PukallR et al.Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol2007; 57:1424–1428
KimuraM. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol1980; 16:111–120 [View Article] [PubMed]
LiR, ZhuH, RuanJ, QianW, FangX et al. De novo assembly of human genomes with massively parallel short read sequencing. Genome Res2010; 20:265–272 [View Article] [PubMed]
KatohK, MisawaK, KumaK, MiyataT. Mafft: a novel method for rapid multiple sequence alignment based on fast fourier transform. Nucleic Acids Res2002; 30:3059–3066 [View Article] [PubMed]
CastresanaJ. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol2000; 17:540–552 [View Article] [PubMed]
StamatakisA. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics2014; 30:1312–1313 [View Article] [PubMed]
RichterM, Rosselló-MóraR. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA2009; 106:19126–19131 [View Article] [PubMed]
ChunJ, OrenA, VentosaA, ChristensenH, ArahalDR et al. Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol2018; 68:461–466 [View Article] [PubMed]
RichterM, Rosselló-MóraR. Shifting the genomic gold standard for the prokaryotic species definition. Proc Natl Acad Sci USA2009; 106:19126–19131 [View Article] [PubMed]
XuP, LiWJ, TangSK, ZhangYQ, ChenGZ et al.Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family Oxalobacteraceae isolated from China. Int J Syst Evol Microbiol2005; 55:1149–1153
WilliamsST, GoodfellowM, AldersonG. Genus Streptomyces Waksman and Henrici 1943, 339AL. WilliamsS, SharpeM, HoltJ. eds In Bergey’s Manual of Systematic Bacteriology Vol 4 Baltimore, MD: Williams and Willkins; 1989 pp 2453–2492
CollinsMD, JonesD. Lipids in the classification and identification of coryneform bacteria containing peptidoglycani based on 2,4-diaminobutyric acid. Appl Bacteriol1980; 48:459–470
MinnikinDE, CollinsMD, GoodfellowM. Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol1979; 47:87–95 [View Article]
CollinsMD, PirouzT, GoodfellowM, MinnikinDE. Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol1977; 100:221–230 [View Article] [PubMed]
TangSK, WangY, ChenY, LouK, CaoLL et al.Zhihengliuella alba sp. nov., and emended description of the genus Zhihengliuella. Int J Syst Evol Microbiol2009; 59:2025–2032 [View Article] [PubMed]