A Gram-stain-negative, aerobic, non-motile, rod-shaped bacterial strain (CAU 1508T) was isolated from marine sediment collected in the Republic of Korea. Growth was observed at 10–45 °C (optimum, 30 °C), pH 4.0–11.0 (optimum, pH 6.0–8.0) and with 0–8.0 % (w/v) NaCl (optimum, 2–4 %). The isolate formed a monophyletic clade in the phylogenetic analyses using 16S rRNA gene and whole-genome sequences, exhibiting the highest similarity to Chachezhania antarctica SM1703T (96.5 %), and representing a distinct branch within the genus Chachezhania (family Rhodobacteraceae). Its whole genome sequence was 5.59 Mb long, with a G+C content of 65.7 mol% and 2183 predicted genes belonging to six functional categories. The average nucleotide identity and digital DNA–DNA hybridization values between CAU 1508T and C. antarctica SM1703T were 79.1 and 22.2 %, respectively. The predominant cellular fatty acids were C19 : 0 cyclo ω8c and summed feature 8 (C18 : 1 ω7c/C18 : 1 ω6c). The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, two unidentified phospholipids and one unidentified aminophospholipid. The sole isoprenoid quinone was ubiquinone 10. Phenotypic phylogenetic properties supported the classification of CAU 1508T as representing a novel species of the genus Chachezhania, with the proposed name Chachezhania sediminis sp. nov. The type strain is CAU 1508T (=KCTC 62999T=NBRC 113697T).
YuanX-X, WangN, ZhangM-Y, ChenX-L, LiC-Y et al.Chachezhania antarctica gen. nov., sp. nov., a novel member of the family ‘Rhodobacteraceae’ isolated from Antarctic seawater. Antonie van Leeuwenhoek2019; 112:1841–1848
LarkinMA, BlackshieldsG, BrownNP, ChennaR, McGettiganPA et al. Clustal W and Clustal X version 2.0. Bioinformatics2007; 23:2947–2948 [View Article] [PubMed]
YoonS-H, HaS-M, KwonS, LimJ, KimY et al. Introducing EzBioCloud: a taxonomically united database of 16S rRNA and whole genome assemblies. Int J Syst Evol Microbiol2017; 67:1613–1617
LeeI, KimYO, ParkSC, ChunJ. OrthoANI: an improved algorithm and software for calculating average nucleotide identity. Int J Syst Evol Microbiol2016; 66:1100–1103
AuchAF, Von JanM, KlenkH-P, GökerM. Digital DNA–DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. Stand Genomic Sci2010; 2:117–134
KimM, OhH-S, ParkS-C, ChunJ. Towards a taxonomic coherence between average nucleotide identity and 16S rRNA gene sequence similarity for species demarcation of prokaryotes. Int J Syst Evol Microbiol2014; 64:346–351
BowmanJP. Description of Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassifcation of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Int J Syst Evol Microbiol2000; 50:1861–1868 [View Article]
Rodríguez-ValeraF, Ruiz-BerraqueroF, Ramos-CormenzanaA. Characteristics of the heterotrophic bacterial populations in hypersaline environments of different salt concentrations. Microb Ecol1981; 7:235–243 [View Article] [PubMed]
SmibertRM, KriegNR. Phenotypic characterization. GerhardtP, MurrayR, WoodW, KriegN. eds In Methods for General and Molecular Bacteriology Washington, DC: American Society for Microbiology; 1994 pp 607–654
CollinsMD, JonesD. A note on the separation of natural mixtures of bacterial ubiquinones using reverse-phase partition thin layer chromatography and high performance liquid chromatography. J Appl Bacteriol1981; 51:129–134 [View Article] [PubMed]