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Volume 71, Issue 7

sp. nov. isolated from internal organs of apparently healthy rainbow trout No Access

Abstract

Two yellow-pigmented isolates, F-60 and F-392, were isolated from the internal organs of an apparently healthy rainbow trout (). The strains were identified as members of the genus based on the results of 16S rRNA gene sequence analysis. Strains F-60 and F-392 had the highest 16S rRNA gene sequence identity level of 97.4 % to the type strain of LPB0076. A polyphasic taxonomic approach including phenotypic, chemotaxonomic and genomic characterization was employed to ascertain the taxonomic position of the strains within the genus . Digital DNA–DNA hybridization (dDDH) and average nucleotide identity based on (ANIb) values for strains F-60 and F-392 were calculated as 100 %. However, dDDH and ANI analyses between the strains and their close neighbours confirmed that both strains represent a novel species in the genus . The strains shared the highest dDDH and ANIb levels of 23.3 and 77.9%, respectively, with the type strain of DSM 17623 while those values for LPB0076 were obtained as 21.4–21.5 % and 76.3 %. The DNA G+C content of the strains was 34.5 mol%. Chemotaxonomic and phylogenomic analyses of these isolates confirmed that both strains are representatives of a novel species for which the name sp. nov. is proposed, with F-60 as the type strain (=JCM 34196=KCTC 82256).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Aquaculture , Flavobacterium , genome analysis and rainbow trout
Funding
This study was supported by the:
  • The Research Fund of Erciyes University (Award TSA-2020-9733)
    • Principle Award Recipient: MuhammedDuman
© 2021 The Authors
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2021-07-23
2024-04-25
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Flavobacterium muglaense sp. nov. isolated from internal organs of apparently healthy rainbow trout
Int J Syst Evol Microbiol 71, 004903 (2021); https://doi.org/10.1099/ijsem.0.004903
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