1887

Abstract

Three novel actinomycete strains, designated as DR6-1, DR6-2 and DR6-4, isolated from the roots of Lindl in Thailand were studied using a polyphasic taxonomic approach. The strains grew at 20–37 °C, at pH 5–10 and with 5 % (w/v) NaCl. They contained -diaminopimelic acid in the cell-wall peptidoglycan and MK-9(H) was a major menaquinone. Arabinose and galactose were the major sugars in the cell wall. The predominant cellular fatty acids were iso-C and iso-C. The detected polar lipids were diphosphatidylglycerol, hydroxyphosphatidylethanolamine, phosphatidylethanolamine, phosphatidylinositol and phosphatidylglycerol. Strains DR6-1, DR6-2 and DR6-4 shared 99.9–100 % 16S rRNA gene sequence similarity and were closely related to JCM 21831 (98.7-98.8%). The approximate genome size of strain DR6-1 was 9.6 Mb with a G+C content of 69.6 mol%. The ANIb and dDDH values between genomic sequences of strain DR6-1 and JCM21831, JCM 10871 and KCTC 39515 were 90.55, 92.25, 92.60%, and 47.20, 52.10 and 52.50%, respectively. Based on the phenotypic, chemotaxonomic and genotypic characteristics, it has been concluded that strains DR6-1, DR6-2 and DR6-4 represent a novel species of the genus for which the name sp. nov. is proposed. The type strain is DR6-1 (=JCM 33742=KCTC 49546=TISTR 2840).

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2021-07-21
2021-07-29
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