1887

Abstract

A polyphasic taxonomic approach was used to characterize a Gram-stain-negative bacterium, designated strain CC-YST696, harbouring antibiotic- and toxic compound-resistace genes, isolated from poultry manure in Taiwan. Cells of CC-YST696 were short rods, motile with polar flagella, catalase- and oxidase-positive. Optimal growth occurred at 30 °С, pH 9 and with 1 % NaCl. The results of phylogenetic analyses based on 16S rRNA genes revealed a distinct taxonomic position attained by CC-YST696 associated with (97.9 % sequence identity), (97.3 %) and (97.2 %), and with lower sequence similarity values to other species. Average nucleotide identity (ANI) values were 72.8–80.0 % (=17) compared within the type strains of species of of the genus . CC-YST696 contained C, C, Cω7 11-methyl and Cω6/ Cω7 as the predominant fatty acids. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids, three unidentified glycolipids, two unidentified phospholipids and three unidentified lipids. The DNA G+C content was 62.2 mol% and the predominant quinone was ubiquinone Q-10. On the basis of its distinct phylogenetic, phenotypic and chemotaxonomic traits together with results of comparative 16S rRNA gene sequence and ANI analyses, strain CC-YST696 is proposed to represent a novel species of the genus , for which the name sp. nov. (type strain CC-YST696=BCRC 81284=JCM 34167) is proposed.

Funding
This study was supported by the:
  • Ministry of Science and Technology, Taiwan (Award MOST 109-2634-F-005-002)
    • Principle Award Recipient: Chiu-ChungYoung
  • Ministry of Science and Technology, Taiwan (Award MOST 109-2634-F-005-002)
    • Principle Award Recipient: Chia-FangTsai
  • Ministry of Science and Technology, Taiwan (Award MOST 109-2634-F-005-002)
    • Principle Award Recipient: Shih-YaoLin
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2021-07-21
2021-07-31
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