1887

Abstract

Two halophilic archaeal strains, Gai3-2 and NJ-3-1, were isolated from salt lake and saline soil samples, respectively, collected in PR China. The 16S rRNA gene sequences of the two strains were 97.5% similar to each other. Strains Gai3-2 and NJ-3-1 had the highest sequence similarities to ‘’ G22 (96.7 and 97.8%, respectively), and displayed similarities of 91.5–93.5% and 92.3–94.7%, respectively, to members. Phylogenetic analysis revealed that the two strains formed different branches and clustered tightly with ‘’ G22 and members. The average nucleotide identity (ANI), in silico DNA–DNA hybridization (DDH) and amino acid identity (AAI) values between the two strains were 83.1, 26.9 and 77.9%, respectively, much lower than the threshold values proposed as a species boundary. These values between the two strains and ‘’ G22 (ANI 77.9–78.2%, DDH 22.5–22.6% and AAI 68.8–69.3%) and members (ANI 77.53–77.63%, DDH 21.8–22.3% and AAI 68.4–69.4%) were almost identical, and much lower than the recommended threshold values for species delimitation. These results suggested that strains Gai3-2 and NJ-3-1 represent two novel species of . Based on phenotypic, chemotaxonomic and phylogenetic properties, strains Gai3-2 (=CGMCC 1.16080=JCM 33550) and NJ-3-1 (=CGMCC 1.16040=JCM 33552) represent two novel species of the genus , for which the name sp. nov. and sp. nov. are proposed.

Funding
This study was supported by the:
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2019FY100700)
    • Principle Award Recipient: Heng-LinCui
  • National Science and Technology Fundamental Resources Investigation Program of China (Award 2017FY100302)
    • Principle Award Recipient: Heng-LinCui
  • National Natural Science Foundation of China (Award 32070003)
    • Principle Award Recipient: Heng-LinCui
  • National Natural Science Foundation of China (Award 31770005)
    • Principle Award Recipient: Heng-LinCui
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2021-07-20
2021-07-31
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