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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 7

sp. nov., isolated from aquaculture water No Access

Abstract

A novel Gram-stain-negative and rod-shaped bacterial strain, designated as 4Y14, was isolated from aquaculture water and characterized by using a polyphasic taxonomic approach. Strain 4Y14 was found to grow at 10–40 °C (optimum, 28 °C), at pH 7.0–9.0 (optimum, 7.0–8.0) and with 0–2 % NaCl (optimum, 1 %, w/v). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 4Y14 belonged to the genus with high levels of similarity to c1 (97.8 %) and c14 (97.2 %). Phylogenomic analysis indicated that strain 4Y14 formed an independent branch distinct from the two type strains above. Digital DNA–DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain 4Y14 and the two type strains were, respectively, 25.3 and 25.0 %, and 81.2 and 80.3 %, which were well below the thresholds of 70 % DDH and 95–96 % ANI for species definition, implying that strain 4Y14 should represent a novel genospecies. The predominant cellular fatty acids of strain 4Y14 were summed feature 3 (C c and/or C c) and iso-C; the major polar lipids were diphosphatidylglycerol, phosphatidylcholine and phosphatidylethanolamine; and the sole respiratory quinone was Q-8. The genomic DNA G+C content was 60.1 mol%. Based on the phenotypic and genotypic analyses, strain 4Y14 is concluded to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of the species is 4Y14 (=GDMCC 1.1934=KACC 22080).

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Chitinilyticum piscinae , genomic analysis , genotypes and phenotypes and polyphasic taxonomy
Funding
This study was supported by the:
  • Science and Technology Plan Project of Guangdong Province (Award 2019B030316017)
    • Principle Award Recipient: Ming-RongDeng
  • GDAS' Project of Science and Technology Development (Award 2020GDASYL-20200103028)
    • Principle Award Recipient: JuanDu
  • Key-Area Research and Development Program of Guangdong Province (Award 2018B020205003)
    • Principle Award Recipient: HonghuiZhu
© 2021 The Authors
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2021-07-26
2024-04-24
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Chitinilyticum piscinae sp. nov., isolated from aquaculture water
Int J Syst Evol Microbiol 71, 004899 (2021); https://doi.org/10.1099/ijsem.0.004899
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