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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 71, Issue 7

sp. nov., a carotenoid-producing bacterium isolated from beach sand No Access

Abstract

A Gram-stain-negative, aerobic and non-motile bacterium, strain sand1-3, was isolated from beach sand collected from Haeundae Beach located in Busan, Republic of Korea. Based on the results of 16S rRNA gene sequence and phylogenetic analyses, CH15-11 (97.0 %), DAC4 (96.8 %), AE3 (96.5 %) and YC6722 (96.0 %) were selected for comparing phenotypic and chemotaxonomic characteristics. Cells of strain sand1-3 grew at 7–50 °C (optimum, 30–35 °C), pH 5.0–8.0 (optimum, pH 7.0–8.0) and in the presence of 0–0.5 % (w/v) NaCl (optimum, 0 %). Major polar lipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid, one unidentified glycolipid and one unidentified phosphoglycolipid. The major fatty acids were summed feature 8 (C ω6 and/or C ω7) and C 2-OH. Moreover, the sole respiratory quinone and major polyamine were identified as ubiquinone-10 and homospermidine, respectively. The genomic DNA G+C content was 65.9 mol%. The digital DNA–DNA hybridization, average nucleotide identity and average amino acid identity values of strain sand1-3 and its reference strains with publicly available genomes were 17.9–18.9 %, 72.0–75.3 % and 63.3–76.5 % respectively. Based on polyphasic evidence, we propose sp. nov. as a novel species within the genus . The type strain is sand1-3 (=KCTC 82358=NBRC 114538).

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Keyword(s): beach sand , carotenoids , phylogenetic analysis , Sphingomonadaceae and Sphingomonas
© 2021 The Authors
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2021-07-29
2024-04-23
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Sphingomonas sabuli sp. nov., a carotenoid-producing bacterium isolated from beach sand
Int J Syst Evol Microbiol 71, 004896 (2021); https://doi.org/10.1099/ijsem.0.004896
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