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Abstract

A novel extreme thermophilic and piezophilic chemoorganoheterotrophic archaeon, strain EXT12c, was isolated from a hydrothermal chimney sample collected at a depth of 2496 m at the East Pacific Rise 9° N. Cells were strictly anaerobic, motile cocci. The strain grew at NaCl concentrations ranging from 1 to 5 % (w/v; optimum, 2.0%), from pH 6.0 to 7.5 (optimum, pH 6.5–7.0), at temperatures between 60 and 95 °C (optimum, 80–85 °C), and at pressures from 0.1 to at least 50 MPa (optimum, 30 MPa). Strain EXT12c grew chemoorganoheterotrophically on complex proteinaceous substrates. Its growth was highly stimulated by the presence of elemental sulphur or -cystine, which were reduced to hydrogen sulfide. The DNA G+C content was 54.58 mol%. Phylogenetic analyses based on 16S rRNA gene sequences and concatenated ribosomal protein sequences showed that strain EXT12c falls into the genus and is most closely related to strain 30-1. Overall genome relatedness index analyses (average nucleotide identity scores and DNA–DNA hybridizations) showed a sufficient genomic distance between the new genome and the ones of the type strains for the delineation of a new species. On the basis of genotypic and phenotypic data, strain EXT12c is considered to represent a novel species, for which the name sp. nov. is proposed, with the type strain EXT12c (=UBOCC M-2417=DSM 111004).

Funding
This study was supported by the:
  • Sino-French IRP 1211 MicrobSea
    • Principle Award Recipient: KarineAlain
  • LabexMER Axis 3 (France) (Award ANR-10-LABX-1)
    • Principle Award Recipient: KarineAlain
  • MERLIN program Abyss (France)
    • Principle Award Recipient: KarineAlain
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2021-07-16
2021-08-04
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