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Volume 71, Issue 7

‘ Xiphinematincola pachtaicus' gen. nov., sp. nov., an endosymbiotic bacterium associated with nematode species of the genus (Nematoda, Longidoridae) Open Access

Abstract

An intracellular bacterium, strain IAS, was observed to infect several species of the plant-parasitic nematode genus (, , , , and ). The bacterium could not be recovered on axenic medium. The 16S rRNA gene sequence of IAS was found to be new, being related to the family Burkholderiaceae, class Betaproteobacteria. Fungal endosymbionts B1-EB (92.9 % sequence identity) and ‘ Glomeribacter gigasporarum’ BEG34 (89.8 % identity) are the closest taxa and form a separate phylogenetic clade inside Burkholderiaceae. Other genes (, and ) also separated this species from its closest relatives using a multilocus sequence analysis approach. These genes were obtained using a partial genome of this bacterium. The localization of the bacterium (via light and fluorescence hybridization microscopy) is in the females clustered around the developing oocytes, primarily found embedded inside the epithelial wall cells of the ovaries, from where they are dispersed in the intestine. Transmission electron microscopy (TEM) observations supported the presence of bacteria inside the nematode body, where they occupy ovaries and occur inside the intestinal epithelium. Ultrastructural analysis of the bacterium showed cells that appear as mostly irregular, slightly curved rods with rounded ends, 0.8–1.2 µm wide and 2.5–6.0 µm long, possessing a typical Gram-negative cell wall. The peptidoglycan layer is, however, evident only occasionally and not detectable by TEM in most cells. Another irregularly occurring shell surrounding the endosymbiont cells or the cell clusters was also revealed, probably originating from the host cell membrane. Flagella or spore-like cells do not occur and the nucleoid is diffusely distributed throughout the cell. This endosymbiont is transmitted vertically through nematode generations. These results support the proposal of IAS as a new species, although its obligate intracellular and obligate endosymbiont nature prevented isolation of a definitive type strain. Strain IAS is therefore proposed as representing ‘ Xiphinematincola pachtaicus’ gen. nov., sp. nov.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Betaproteobacteria , intracellular micro-organism , maternal transmission , MLSA and Xiphinema
Funding
This study was supported by the:
  • Ministerio de Ciencia, Innovación y Universidades (Award RTI2018-095925-A-100)
    • Principle Award Recipient: JuanEmilio Palomares-Rius
  • Consejo Superior de Investigaciones Científicas (Award 201740E042)
    • Principle Award Recipient: P.Castillo
© 2021 The Authors
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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2021-07-21
2024-04-25
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‘Candidatus Xiphinematincola pachtaicus' gen. nov., sp. nov., an endosymbiotic bacterium associated with nematode species of the genus Xiphinema (Nematoda, Longidoridae)
Int J Syst Evol Microbiol 71, 004888 (2021); https://doi.org/10.1099/ijsem.0.004888
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