1887

Abstract

A Gram-stain-negative, oxidase-positive, catalase-positive, aerobic, orange-pigmented, rod-shaped and non-motile bacterium designated strain MMS17-SY002 was isolated from island soil. The isolate grew at 20–37 °C (optimum, 30 °C), at pH 6.0–9.5 (optimum, pH 7) and in the presence of 0.5–4.0 % (w/v) NaCl (optimum, 2.0 %). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain MMS17-SY002 was mostly related to the genus of the family and had highest sequence similarity of 96.82 % to A6B8 and EM44, but formed a distinct phylogenetic line within the genus. Chemotaxonomic analyses showed that menaquinone 6 was the predominant isoprenoid quinone, the major fatty acids were iso-C G and iso-C, and the diagnostic polar lipid was phosphatidylethanolamine. The genomic DNA G+C content was 42.4 mol%. Strain MMS17-SY002 could be distinguished from related species by the combination of trypsin, α-chymotrypsin, acid phosphatase, naphthol-AS-BI-phosphohydrolase, α-galactosidase, β-galactosidase and β-glucosidase activities. The orthologous average nucleotide identity between the genomes of strain MMS17-SY002 and and that between the strain and A6B8 were 73.26 and 73.33%, respectively, thus confirming the separation of the strain from related species at species level. Based on the phenotypic, phylogenetic, chemotaxonomic and genomic characterization, MMS17-SY002 should be recognized as a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MMS17-SY002 (=KCTC 62790=JCM 32370).

Funding
This study was supported by the:
  • National Institute of Biological Resources
    • Principle Award Recipient: SeungBum Kim
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijsem.0.004887
2021-07-16
2024-03-29
Loading full text...

Full text loading...

References

  1. Kahng H-Y, Lee S-S, Kim JM, Jung JY, Lee MY et al. Muriicola jejuensis gen. nov., sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2010; 60:1644–1648 [View Article]
    [Google Scholar]
  2. Hu J, Zhang WY, Zhang XQ, Zhu XF, Wu M. Muriicola marianensis sp. nov., isolated from seawater. Int J Syst Evol Microbiol 2015; 65:407–411 [View Article]
    [Google Scholar]
  3. Bernardet JF, Nakagawa Y, Holmes B. Subcommittee on the Taxonomy of Flavobacterium and Cytophaga-like Bacteria of the International Committee on Systematics of Prokaryotes Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. Int J Syst Evol Microbiol 2002; 52:1049–1070 [View Article]
    [Google Scholar]
  4. Jooste P, Hugo CJ. The taxonomy, ecology and cultivation of bacterial genera belonging to the family Flavobacteriaceae . Int J Food Microbiol 1999; 53:81–94 [View Article]
    [Google Scholar]
  5. Alain K, Intertaglia L, Catala P, Lebaron P. Eudoraea adriatica gen. nov., sp. nov., a novel marine bacterium of the family Flavobacteriaceae . Int J Syst Evol Microbiol 2008; 58:2275–2281 [View Article]
    [Google Scholar]
  6. Asker D, Beppu T, Ueda K. Zeaxanthinibacter enoshimensis gen. nov., sp. nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae, isolated from seawater off Enoshima Island, Japan. Int J Syst Evol Microbiol 2007; 57:837–843 [View Article]
    [Google Scholar]
  7. Kim MK, Kang HJ, Roh SG, Park JS, Kim SB. Streptomyces fodineus sp. nov., an actinobacterium with antifungal activity isolated from mine area soil. Int J Syst Evol Microbiol 2019; 69:1350–1354 [View Article]
    [Google Scholar]
  8. Jeon Y-S, Lee K, Park S-C, Kim B-S, Cho Y-J et al. EzEditor: a versatile sequence alignment editor for both rRNA- and protein-coding genes. Int J Syst Evol Microbiol 2014; 64:689–691 [View Article]
    [Google Scholar]
  9. Kumar S, Stecher G, Tamura K. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 2016; 33:1870–1874 [View Article]
    [Google Scholar]
  10. Fitch WM. Toward defining the course of evolution: minimum change for a specific tree topology. Syst Biol 1971; 20:406–416 [View Article]
    [Google Scholar]
  11. Felsenstein J. Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 1981; 17:368–376 [View Article]
    [Google Scholar]
  12. McBride MJ, Zhu Y. Gliding motility and por secretion system genes are widespread among members of the Phylum Bacteroidetes . Int J Bacteriol 2012JB.296 01962-01912
    [Google Scholar]
  13. Kovacs N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature 1956; 178:703 [View Article]
    [Google Scholar]
  14. Baumann P, Moran N, Baumann L. The Prokaryotes, a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Applications New York: Springer; 2000
    [Google Scholar]
  15. Cowan ST. Cowan and Steel’s Manual for the Identification of Medical Bacteria Cambridge University Press; 2004
    [Google Scholar]
  16. Asker D, Beppu T, Ueda K. Zeaxanthinibacter enoshimensis gen. Nov., sp. Nov., a novel zeaxanthin-producing marine bacterium of the family Flavobacteriaceae, isolated from seawater off Enoshima Island, Japan. Int J Syst Evol Microbiol 2007; 57:837–843 [View Article]
    [Google Scholar]
  17. Sasser M. Identification of Bacteria by Gas Chromatography of Cellular Fatty Acids, MIDI Technical Note 101. Newark, DE, USA: Microbial ID Inc; 1990
    [Google Scholar]
  18. Kim MJ, Roh SG, Kim M-K, Park C, Kim S et al. Kitasatospora acidiphila sp. Nov., isolated from pine grove soil, exhibiting antimicrobial potential. Int J Syst Evol Microbiol 2020; 70:5567–5575 [View Article]
    [Google Scholar]
  19. Minnikin D, O’donnell A, Goodfellow M, Alderson G, Athalye M et al. An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 1984; 2:233–241 [View Article]
    [Google Scholar]
  20. Yoon S-H, Ha S-M, Lim JM, Kwon SJ, Chun J. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 2017; 110:1281–1286 [View Article]
    [Google Scholar]
  21. Meier-Kolthoff JP, Auch AF, Klenk HP, Göker M. Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinformatics 2013; 14:60 [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijsem.0.004887
Loading
/content/journal/ijsem/10.1099/ijsem.0.004887
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error