1887

Abstract

Two Gram-stain-negative, yellow-pigmented and strictly aerobic bacteria, designated strains SE-s27 and SE-s28, were isolated from forest soil. Both strains were non-motile rods that were catalase-positive and oxidase-negative and grew optimally at 25–30 °C, pH 8.0 and with 0 % (w/v) NaCl. Strain SE-s28 produced flexirubin-type pigments, but strain SE-s27 did not produce them. Both strains contained menaquinone-6 as the sole respiratory quinone and phosphatidylethanolamine as a major polar lipid. As the major cellular fatty acids (>10 %), SE-s27 contained iso-C and iso-CG, whereas SE-s28 contained iso-C and summed feature 3 comprising Cωc and/or Cωc and/or iso-C 2-OH. The DNA G+C contents of strains SE-s27 and SE-s28 were 33.1 and 44.3 mol%, respectively. The results of phylogenetic analysis based on 16S rRNA gene sequences revealed that SE-s27 and SE-s28 formed respective distinct phylogenetic lineages within the genus . Strains SE-s27 and SE-s28 were most closely related to an-8 and ICH-30 with 98.0 and 94.5 % 16S rRNA gene sequence similarities, respectively. In conclusion, strains SE-s27 and SE-s28 represent novel species of the genus , for which the names sp. nov. and sp. nov. are proposed. The type strains of and are SE-s27 (=KACC 18802=JCM 31544) and SE-s28 (=KACC 18803=JCM 31545), respectively.

Funding
This study was supported by the:
  • National Institute of Biological Resources (Award NIBR No. 2020-02-001)
    • Principle Award Recipient: CheOk Jeon
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2021-07-13
2021-07-29
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